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JoVE Journal

Medicine

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Polyacrylamide Gels for Invadopodia and Traction Force Assays on Cancer Cells

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08:48 min

January 4th, 2015

January 4th, 2015

11,894 Views

0:05

Title

1:22

Preparation of Glass Coverslips for Polyacrylamide Gels

2:59

Preparation of Polyacrylamide Gels for Invadopodia Assays

5:09

Preparation of Extracellular Matrix for Invadopodia Assays

7:10

Results: Invadopodia and Traction Force Assays

8:24

Conclusion

Mechanical rigidity in the tumor microenvironment plays a crucial role in driving malignant behavior by increasing invadopodia activity and actomyosin contractility. Using polyacrylamide gels (PAAs), invadopodia and traction force assays can be utilized to study the invasive and contractile properties of cancer cells in response to matrix rigidity.

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