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08:46 min
January 6th, 2015
DOI :
10.3791/52357-v
Chapters
0:05
Title
1:58
Analysis of Isopropyl Beta-D-1-thiogalactopyranoside (IPTG)-induced Expression by In-gel Flourescence
3:37
Scale-up of Cell Cultures and Membrane Preparation
5:30
Solubilization and Analysis of the Membrane Fraction
6:48
Results: Representative In-gel and FSEC Analyses
8:11
Conclusion
Summary
녹색 형광 단백질에 융합을 기반으로 대장균에서 재조합 막 단백질의 발현에 대한 심사에 대한 효율적인 접근 방법이 제시된다.
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