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Chemistry

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Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

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09:49 min

April 2nd, 2015

April 2nd, 2015

10,461 Views

0:05

Title

1:48

Density Matching of Detergent Miscelles with 2H2O

5:37

Sedimentation Equilibrium of SH Protein in C14 Sulfobetaine Micelles

8:30

Results: Effect of pH on SH Protein Pentameric Stability

9:08

Conclusion

Sedimentation equilibrium (SE) can be used to study protein-protein interactions in a physiological environment. This manuscript describes the use of this technique to determine the effect of pH on the stability of a homo-pentamer formed by the small hydrophobic (SH) protein encoded by the human syncytial respiratory virus (hRSV).

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