RNA and DNA Isolation from an Enriched Astrocytic Population using FACS
4:03
Assessing DNA Methylation Status of a Gene Using Methylation-sensitive High Resolution Melt Analysis
5:26
Assessing Hyper-methylated Promoter Activity using a Luciferase Assay
8:50
Results: DNA Methylation Analysis of KCNJ10 in Astrocytes
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Conclusion
DNA methylation is capable of maintaining stable levels of gene expression as well as allowing for dynamic changes in gene expression in response to a variety of stimuli. We detail techniques that allow the study of gene-specific changes in DNA methylation and the effect of these changes on gene expression.