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DOI :
10.3791/52458-v
•
10:41 min
March 17th, 2015
Chapters
0:05
Title
1:52
Preparation of Dissection and Culture Media
3:44
Neonatal Ovary Dissection and Culture
5:32
Follicle Dissection, Culture and Co-culture
8:49
Results: Environmental Effects on Ovary and Follicle Cell Growth
10:05
Conclusion
このプロトコルは、新生児マウスと思春期前のマウスからの個々の卵胞から卵巣を使って、マウスの卵巣組織の初代培養/共培養を説明しています。培養技術は、卵巣上の外因性薬剤の効果の研究を可能にする、高度に生理的な方法で開発を支援し、卵胞の間の相互作用の。
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