Preparation of Single Cell Suspensions from Human Skin
4:54
Polyclonal Activation of Skin-resident T Cells and Flow Cytometry Analysis
8:04
Results: Phenotypic Analysis of Skin-derived Lymphocytes
10:10
Conclusion
Transcript
The overall goal of this procedure is to efficiently isolate skin resident T cells from human skin biopsies. This is accomplished by first obtaining human skin biopsies using a round biopsy punch. Next, each skin biopsy is cut into four smaller pi
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We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.