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10.2K Views
•
09:05 min
May 6th, 2015
DOI :
10.3791/52588-v
Chapters
0:05
Title
1:59
Eye Enucleation and Isolation of Retina
3:22
Slice Preparation
4:59
Two-photon Microscopy
6:14
Light Stimulation and Recording
6:53
Results: Light-evoked Ca2+ Responses in Axon Terminals of Mouse Cone Photoreceptors
8:10
Conclusion
Summary
我们描述了一个协议,以监控使用前体切片准备小鼠视网膜锥感光细胞的轴突终末钙离子动力。该协议允许锥钙离子信号的一个重要哺乳动物模型系统全面的研究,鼠标。
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