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10.2K Views
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09:05 min
May 6th, 2015
DOI :
10.3791/52588-v
Chapters
0:05
Title
1:59
Eye Enucleation and Isolation of Retina
3:22
Slice Preparation
4:59
Two-photon Microscopy
6:14
Light Stimulation and Recording
6:53
Results: Light-evoked Ca2+ Responses in Axon Terminals of Mouse Cone Photoreceptors
8:10
Conclusion
Summary
我々は、マウス網膜のex vivoでのスライス標本を用いて、錐体光受容体の軸索末端におけるCa 2+動態を監視するためのプロトコルを記述します。このプロトコルは、重要な哺乳動物モデル系におけるコーンのCa 2+シグナル伝達の包括的研究、マウスを許可します。
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