Name: demonstration of the rat ischemic skin wound model
Uploaded: 2015-04-01T15:00:00
Description: Watch this Scientific Journal Video about Demonstration of the Rat Ischemic Skin Wound Model at JoVE.com

The authors would like to acknowledge funding support from the University of South Florida, Department of Surgery (Mack Wu, MD, PhD).

来自南佛罗里达州的动物护理委员会（IACUC）的大学，获得批准​​下文提出的所有动物的程序，并通过动物福利法和指南护理和使用实验动物的所有要求遵守：注意。 1.准备硅床单和手术器械<ol><li>预切割的条带（10.5厘米×3.0cm）的非增强0.01厚度，医用级硅酮薄片和消毒使用高压釜。 </li><li>清洁和消毒适合手术器械（剪刀，镊子和窗帘或毛巾在手术过程中创造一个无菌区?...

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R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Altered+collagen+metabolism+and+delayed+healing+in+a+novel+model+of+ischemic+wounds.">Altered collagen metabolism and delayed healing in a novel model of ischemic wounds.</a> Wound Repair Regen. 3 (2), 204-212 (1995).</li><li>Chen, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+and+mechanistic+validation+of+delayed+healing+rat+wounds+as+a+model+for+human+chronic+wounds.">Molecular and mechanistic validation of delayed healing rat wounds as a model for human chronic wounds.</a> Wound Repair and Regeneration. 7, (1999).</li><li>Zhang, Q., Chang, Q., Cox, R. A., Gong, X., Gould, L. 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<html>大鼠伤口愈合经常被讨论的主题，因为它们愈合伤口感染和动物间差异5率高的能力。它的一个发展过程中的模型的最初目标是要减少这种变化。修改到护翼的宽度，减少伤口放置特定的数量（集中在具有一致的头尾位置的翼片）的硅酮片材的和引入已经完成了这个目标。通过收缩伤口愈合也已减少和治疗上皮，如在人类中，是测得的结果。该模型以不同的菌株鼠适应， 即 F344?...

有效的药物开发等伤口愈合的治疗需要适当的体内模型，尽管翻译在动物模型的研究结果为人类治疗1已知问题。以下是对使用局部缺血皮肤伤口愈合的大鼠模型的详细协议的描述，调查机制的病理伤口愈合进一步的理解。鼠种，经常采用，由于其广泛的可用性，尺寸和用于驯服自然伤口愈合研究，因为它是足够大以提供一个合适的皮肤面积为切口和切除伤人，成像和组织收集2。然而，应当考虑到仔细考虑了大鼠和人的皮肤上是不同的解剖学上，用大鼠被称为松散皮肤动物。这显着特点，使得伤口收缩，而不是上皮到显著到大鼠皮肤闭幕瓦特贡献ounds 2。此外，皮下肉膜肌的大鼠的存在下，通过双方的收缩和胶原形成3,4-有助于愈合。这些非常重要的解剖区分被认为是在大鼠缺血性皮肤伤口模型的发展和具体的修改被实施，以减少伤口收缩，减少肉膜肌5的影响。 在糖尿病足部溃疡，下肢静脉溃疡和压力性溃疡，愈合被延迟和这些伤口被认为是慢性的。伤口的特征在于过度的炎症，其防止从伤口进展到伤口修复6的下一个阶段。一种在慢性伤口的发展的主要因素是局部组织缺血（血流量减少）5有助于无法清除炎症。在这期间这款机型正在开发和验证（2003-2004年中的时候），没有标准化的动物模型，可以提供足够的组织以测试诱导血管生成的伤口床，在正常的伤口愈合的关键阶段和动机发展此模型5。这就是说，这里提出的模型是最初由Schwartz 等7和随后描述的缺血性伤口模型的变形由Chen 等人所用的修饰的形式。8 在修改后的缺血性伤口模型，已经作了修改，以规避的大鼠的上述解剖特征，导致治疗效果的收缩，而不是上皮：（1）两个全层切除伤口内bipedicled背部皮肤挡板和创建肉膜肌从由解剖正上方的肌肉筋膜床伤口移除。 （2）在翼片本身具有位于在t的中点更窄的尺寸，以确保血液供应是随机的，伤口他皮瓣缺血是。 （3）一种硅片被插入挡板下面，以防止readherence从下层组织瓣的再灌注。伤口收缩是有限的（不排除）由固定或缝合皮瓣硅胶片5。 该模型最近已应用在研究范围从对缺血性伤口愈合9,10-缺血伤口愈合高压氧影响在年轻与老年大鼠11，并已被证明是长期组织缺血的一个可靠的模型。在bipedicled瓣的尺寸也已经适应了不同的大鼠品系，包括只SD（11厘米长2厘米宽）和F344大鼠（10.5厘米长按3.0-3.5厘米宽）和其他物种，包括猪12鼠13,14。这部影片采用了缺血性皮肤创伤模型的演示F344近交系大鼠品系。 审批下面提出的所有动物的程序是获得编辑从南佛罗里达州的动物护理委员会（IACUC）的大学和动物福利法案和指南护理和使用实验动物的所有要求遵守。

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demonstration of the rat ischemic skin wound model

在人类的倾向慢性伤口增加了与老化，疾病状况如糖尿病和心血管障碍的功能，并且由于动减轻的压力。动物模型已经开发了试图模仿这些条件促进我们的慢性伤口的复杂性的理解的目的。本文所描述的模型是大鼠缺血皮瓣模型允许长期减少导致伤口成为局部缺血和类似慢性伤口的表型（缩血管化，增加了炎症和延迟伤口闭合）血流。它由一个bipedicled背瓣集中放置2缺血性伤口和2个非缺血性伤口横向到瓣作为对照。一种新型除此之外缺血皮瓣模型是安置在充当屏障，夹板，以防止血管再通，减少收缩的伤口愈合瓣下方的硅胶片的。尽管使用大鼠伤口愈合研究，由于其相比于人类相当不同解剖和生理差异的争论（ 即一个肉膜肌，短寿命的存在下，毛囊的数量增加，并且其愈合伤口感染能力）在这个模型中采用的修改，使其成为一个有价值的替代以前开发的缺血皮瓣模型。

大鼠缺血性伤口愈合模型协议应该采取每头约20分钟，如果有效地执行。此前申请的敷料的模型应该出现如图1G表示。这将是重要的，以验证该bipedicled襟翼和伤口在其中是缺血。皮下氧张力（PSCO 2）在伤口的水平这一模型5的验证期间被测量通过放置一个极谱电极在两个缺血性伤口之间的皮下组织。 PSCO 2值分别在危重缺血范围（20-40毫米汞柱）。由于这种模式?...

Watch this Scientific Journal Video about Demonstration of the Rat Ischemic Skin Wound Model at JoVE.com

Demonstration of the Rat Ischemic Skin Wound Model

鼠，由于它的尺寸，可用性，和相当温顺的行为，已经被用作研究模型多年。该协议的目的是利用老鼠作为缺血性皮肤伤口愈合模型提供有价值的洞察慢性伤口的病理生理学。

在大鼠缺血性皮肤创伤模型的演示

The propensity for chronic wounds in humans increases with ageing, disease conditions such as diabetes and impaired cardiovascular function, and ...

Research

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A Simplified Technique for Producing an Ischemic Wound Model

一个生产缺血性损伤模型的简化技术

One major obstacle in current diabetic wound research is a lack of an ischemic wound model that can be safely used in diabetic animals. Drugs that work ...

科研

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Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

内皮素-1诱导大脑中动脉闭塞模型缺血性中风激光多普勒血流仪在大鼠指导

Stroke is the number one cause of disability and third leading cause of death in the world, costing an estimated $70 billion in the United States in ...

Angiogenesis in the Ischemic Rat Lung

在缺血大鼠肺的血管生成

The  adult lung is perfused by both the systemic bronchial artery and the entire  venous return flowing through the pulmonary arteries. In most lung ...

Murine Model of Wound Healing

伤口愈合的小鼠模型

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. ...

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Experimental saccular aneurysm models are necessary for testing novel surgical and endovascular treatment options and devices before they are introduced ...

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia

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Despite profound expertise and advanced surgical techniques, ischemia-induced complications ranging from wound breakdown to extensive tissue necrosis are ...

A Mouse Fetal Skin Model of Scarless Wound Repair

对无瘢痕修复鼠标胎儿皮肤模型

Early in utero, but not in postnatal life, cutaneous wounds undergo regeneration and heal without formation of a scar. Scarless fetal wound healing occurs ...

Rat Model of Photochemically-Induced Posterior Ischemic Optic Neuropathy

光化学诱导后部缺血性视神经病变的大鼠模型

Posterior Ischemic optic neuropathy (PION) is a sight-devastating disease in clinical practice. However, its pathogenesis and natural history ...

A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy

新生儿缺氧缺血性脑病的小猪模型

Birth asphyxia, which causes hypoxic-ischemic encephalopathy (HIE), accounts for 0.66 million deaths worldwide each year, about a quarter of the ...

The Rodent Model of Nonarteritic Anterior Ischemic Optic Neuropathy (rNAION)

The Rodent Model of Nonarteritic Anterior Ischemic Optic Neuropathy rNAION

非动脉炎性前部缺血性视神经病变的啮齿动物模型（rNAION）

Nonarteritic anterior ischemic optic neuropathy (NAION) is a focal ischemic lesion of the optic nerve that affects 1/700 individuals throughout their ...