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JoVE Journal

Environment

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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

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12:32 min

January 16th, 2016

January 16th, 2016

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Title

2:03

Tertiary Concentration and Nucleic Acid Isolation

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Reverse Transcription

5:57

Real Time Quantitative PCR

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Results: RT-qPCR Determination of Enterovirus and Norovirus in Water

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Conclusion

Transcript

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

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