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Methods for Culturing Human Femur Tissue Explants to Study Breast Cancer Cell Colonization of the Metastatic Niche

12.4K Views

11:41 min

March 15th, 2015

March 15th, 2015

12,388 Views

0:05

Title

2:00

Isolating Tissue Fragments from Femoral Heads

2:40

Co-culturing to Study Cell Proliferation

4:19

Co-culturing to Study Colonization and Cell Number

6:45

Measuring Cell Migration in the Co-culture: Towards Bone Tissue Supernatant

9:30

Results: Breast Cancer Cell Proliferation, Colonization, and Migration in Co-culture

11:10

Conclusion

Protocols are described for studying breast cancer cell migration, proliferation and colonization in a human bone tissue explant model system.

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