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DOI :
10.3791/52691-v
May 1st, 2015
Chapters
0:05
Title
1:14
Foreskin and Lymphatic Malformation Cell Suspension Preparation
4:01
Antibody Staining of Lymphatic Endothelial Cell (LEC) Surface Markers
5:49
Results: Representative Foreskin and Lymphatic Malformation LECs
6:53
Conclusion
このプロトコルの目的は、蛍光活性化セルソーティング(FACS)を用いて、ヒトのリンパ奇形嚢胞様血管および包皮を裏打ちするリンパ管内皮細胞を単離することです。その後の細胞培養およびこれらの細胞の拡大は、遺伝的プロテオミクス、機能および細胞分化の研究のための実験的な洗練の新しいレベルを可能にします。
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