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20:51 min
December 4th, 2007
DOI :
10.3791/527-v
Chapters
0:00
Title
0:10
Introduction
1:06
Cardiac Perfusion
5:36
Removing the Brain
9:11
Removing the Spinal Cord
11:18
Preparation of a Single-Cell Suspension
14:12
Percoll Gradient
17:41
Harvest of Mononuclear Cells
19:56
Conclusion
Summary
In this video we demonstrate how to isolate mononuclear cells from the central nervous system of rats with experimental autoimmune encephalomyelitis.
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