Name: paw-dragging: a novel, sensitive analysis of the mouse cylinder test
Uploaded: 2015-04-29T16:00:00
Description: Watch this Scientific Journal Video about Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test at JoVE.com

نشكر السيد جون كروويل والسيد تيري Upshall للحصول على الخبرة التقنية والمساعدة في التصوير الفوتوغرافي وبالفيديو. وأيد هذا العمل من خلال تشغيل المنح لJLV من المعاهد الكندية لأبحاث الصحة ومؤسسة البحوث والتنمية في نيوفاوندلاند والقلب والسكتة الدماغية مؤسسة كندا الشراكة الكندية للحصول على منحة السكتة الدماغية محفز الانتعاش. كان RBR حاصل على كيث جريفيث التذكارية القلب والسكتة الدماغية مؤسسة العليا للمنح الدراسية.

بيان الأخلاق: تمت الموافقة على جميع التجارب التي كتبها جامعة ميموريال لجنة أخلاقيات رعاية الحيوان نيوفاوندلاند وفقا للمبادئ التوجيهية للمجلس الكندي للرعاية الحيوان. 1. الفئران <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> استخدام فئرا...

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النقاط الرئيسية لإقامة عند قياس سلوك سحب مخلب في اسطوانة الاختبار ما يلي: أ) تحديد عدد مخلب-تستمر مقابل إجمالي اللمسات مخلب لكل مخلب قبل إصابات الدماغ لإنشاء خط الأساس. ب) تحديد عدد من مخلب-المؤثرات السلبية مقابل إجمالي اللمسات مخلب لكل مخلب بعد الإصابة الدماغية. والث...

هدف استراتيجيات تجديد العصبية هو تثبت كل إصلاح الأنسجة والانتعاش وظيفية. وعادة ما يتم تقييم الانتعاش وظيفية مع الاختبارات السلوكية التي تقيس عجز وظيفي، في هذه الحالة التي تنطوي على المهارات الحركية التي ترتبط مع الأضرار التي لحقت مناطق محددة في الدماغ. إصابات في الدماغ أو ضرر نقص تروية إلى منطقة forelimb الحسية من القشرة يمكن البرهنة من قبل عدد من الاختبارات السلوكية. واحدة من هذه التجارب، يتم استخدام اسطوانة الاختبار على نطاق واسع في الفئران لتقييم عجز وظيفي في نشاط forelimb 1. اختبار لديه منخفضة التكلفة انشاء تتطلب سوى اسطوانة، وكاميرا والجدول مع كبار شفافة. فمن السهل أن إدارة لأنه يقوم على السلوك الاستكشافي الطبيعية من القوارض، لذلك ليس هناك حاجة للتدريب قبل والحرمان من الطعام أو المكافآت. وعلى الرغم من هذه المزايا العديدة، ويستخدم تحت الاختبار اسطوانة لتقييم العجز forelimb في الفئران بعد اصابات التنسيق إلى الصورة forelimbensorimotor القشرة، التي نوليها لتحليل سلوك الماوس في اختبار اسطوانة. التباين Forelimb هو المقياس التقليدي للتحليل لاختبار اسطوانة. عندما وضعت في الاسطوانة والقوارض بشكل طبيعي استكشاف جدران الاسطوانة تربية على أطرافهم الخلفية ولمس جدران الاسطوانة مع الكفوف forelimb لتحقيق التوازن الوضعي. يمكن تقدير عدد من اللمسات مخلب مع الجدار مع كل forelimb بسهولة عن طريق تصوير القوارض خلال هذا الاستكشاف للاسطوانة. يحدث عدم تناسق Forelimb عندما يجعل مخلب forelimb المتضررة أقل اللمسات مع الجدار من forelimb مخلب تتأثر ويدل على الأضرار التي لحقت الحسية القشرة المقابل. في الفئران، والحقن داخل القشرية من وكيل مضيق للأوعية، Endothelin (ET-1)، إلى القشرة الحسية forelimb يسبب آفة الدماغية التنسيق مما يؤدي إلى العجز السلوكية في forelimb المقابل. تم الكشف عن العجز في استخدام forelimb المقابل بسهولة عن التغيرات في وorelimb عدم التماثل في اختبار اسطوانة في الفئران 1-3. وعلى النقيض من الفئران ومع ذلك، والتغيرات في forelimb التباين متغيرة وأقل ثباتا في الفئران التالية مقارنة ET-1 حقن 4-6. نحن هنا يبرهن على وجود تحليل رواية السلوك forelimb في اختبار اسطوانة - تحليل السلوك سحب مخلب. لقد أظهرنا سابقا أن تحليل سحب مخلب هو مقياس أكثر حساسية من الأضرار التي لحقت الحسية القشرة forelimb في الفئران من تحليل التباين forelimb الكلاسيكية، وبالتالي ينطبق على مجموعة متنوعة من نماذج التنسيق إصابة القشرية. بحث كيفية الاتصال forepaw جدار الاسطوانة التالية الأضرار الناجمة عن فقر الدم إلى القشرة الحسية forelimb كشف سلوك الرواية في الفئران - مخلب-سحب 4. يحدث مخلب السحب عندما يقف الماوس على سيقانه الخلفية لاستكشاف جدار الاسطوانة ثم تستمر في المتضررين (مضاد التقرحي) مخلب على طول جدار الاسطوانة نحو خط الوسط، أو إلى أسفل بينما الحائطيوفر forepaw لها تتأثر دعم الوضعي ضد الجدار. مخلب-تستمر نادرا ما تحدث في الفئران لم يصب بالتالي ظهور مخلب السحب هو مؤشر إيجابي من إصابة في forelimb الحسية القشرة 4. لقد كميا سابقا السلوك سحب مخلب في الفئران التالية ET-1 الأضرار الدماغية إلى forelimb الحسية القشرة وأظهرت سلوك سحب مخلب المستمر في الفئران تصل إلى أسبوعين بعد السكتة الدماغية 4. نحن هنا تشير إلى أن يستمر السلوك سحب مخلب تصل إلى أربعة أسابيع بعد السكتة الدماغية. تحليل سلوك سحب مخلب يوفر أداة جديدة وحساسة لتقييم الأضرار الناجمة عن فقر الدم البؤري إلى القشرة الحسية forelimb في الفئران. به غير مكلفة الاعداد، وسهولة الإدارة ويحرز جعل هذا بسيط، وأداة ولكنها مفيدة لتقييم بسرعة العجز السلوكية forelimb في الفئران.

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>Plexi-glass cylinder</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>17.5cm high, 9.5cm outer diameter, 8.8cm inner diameter, wall thickness 0.35cm (or 3.5 mm)</td>
		</tr>
		<tr>
			<td>viewing table</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>54x56x66.5cm (width x length x height), top of table is a 51x51cm sheet of plexiglass.</td>
		</tr>
		<tr>
			<td>mirror</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>34x58cm mirror</td>
		</tr>
		<tr>
			<td>video camera</td>
			<td>Sony</td>
			<td>DCR-SR42</td>
			<td>Video camera with onboard storage, SD functionality, 40x optical zoom</td>
		</tr>
		<tr>
			<td>computer</td>
			<td>Dell</td>
			<td>Optiplex 760</td>
			<td>Processor: Intel, 3.0 GHz, Memory 4.00GB (RAM)&#160;</td>
		</tr>
		<tr>
			<td>computer monitor</td>
			<td>Samsung</td>
			<td>S22C350H</td>
			<td></td>
		</tr>
		<tr>
			<td>Excel (Microsoft Office Professional Plus)</td>
			<td>Microsoft</td>
			<td>v14.0.7106.5003</td>
			<td></td>
		</tr>
		<tr>
			<td>VLC Media Player</td>
			<td>Video LAN</td>
			<td>v2.1.2</td>
			<td>Media player with playback speed modulation and video support</td>
		</tr>
		<tr>
			<td>External Hard Drive</td>
			<td>Western Digital</td>
			<td>WDBAAU0020HBK-01</td>
			<td>2 TB</td>
		</tr>
	</tbody>
</table>

paw-dragging: a novel, sensitive analysis of the mouse cylinder test

يتم استخدام اسطوانة الاختبار بشكل روتيني للتنبؤ الأضرار الدماغية البؤري إلى القشرة الحركية forelimb في القوارض. عندما وضعت في الاسطوانة والقوارض تستكشف من قبل تربية ولمس جدران الاسطوانة مع الكفوف forelimb من أجل دعم الوضعي. بعد الإصابة الدماغية إلى القشرة الحسية forelimb، الجرذان تعتمد بشكل أكبر على من لم يتأثر forelimb مخلب لدعم الوضعي مما أدى إلى اللمسات أقل مع مخلب المتضررين والذي يسمى forelimb التباين. في المقابل، فشل الأضرار الدماغية البؤري في مخ الفأر أن يؤدي إلى العجز مقارنة ثابتة في forelimb التباين. في حين عجز forelimb التباين لوحظ بشكل غير منتظم، والفئران لا يبرهن على وجود وظيفة السلوك رواية السكتة الدماغية تسمى &quot;سحب-باو&quot;. مخلب-السحب هو الميل عن الماوس لسحب مخلب المتضررين على طول جدار الاسطوانة بدلا من دفع مباشرة من خارج الجدار عند الترجل من الخلف إلى موقف أربعة أرجل. لقد سبق أثبتت أنمخلب-سحب السلوك حساس جدا للإصابات الدماغية القشرية الصغيرة إلى القشرة الحركية forelimb. نحن هنا نقدم بروتوكول مفصل لتحليل سحب مخلب. نحدد ما هو مخلب السحب وشرح كيفية تحديد سلوك سحب مخلب. اختبار اسطوانة هو اختبار بسيط وغير مكلف لإدارة ولا يتطلب التدريب قبل أو استراتيجيات الحرمان من الطعام. في استخدام تحليل سحب مخلب مع اختبار اسطوانة، يملأ مكانا للتنبؤ الإصابات الدماغية القشرية مثل photothrombosis وEndothelin-1 (ET-1) نقص التروية يسببها - نموذجين التي تزيد على الإطلاق في شعبية وتنتج إصابات التنسيق أصغر من وسط انسداد الشريان الدماغي. أخيرا، وقياس السلوك سحب مخلب في اسطوانة الاختبار سيسمح دراسات الانتعاش وظيفية بعد الاصابة القشرية باستخدام فوج واسعة من سلالات الفئران المعدلة وراثيا حيث فشلت السابق تحليل forelimb التماثل للكشف عن عجز متسقة.

لقد أثبتنا سابقا يبدو أن سلوك سحب مخلب بعد الإصابة الدماغية التنسيق إلى forelimb الحسية القشرة، وهو مؤشر إيجابي من الضرر 4. استخدمت الحقن داخل القشرية من ET-1 في القشرة الحسية forelimb للحث على آفة الدماغية (الشكل 8A، B). وبحثت هذه الدراسة ما إذا كان سلوك سحب مخلب ت?...

Watch this Scientific Journal Video about Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test at JoVE.com

Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test

يستخدم الكلاسيكية تحليل forelimb التماثل للاسطوانة الاختبار بشكل روتيني لتقييم العجز السلوكية في الفئران بعد إصابات الدماغ أو السكتة الدماغية. ومع ذلك، فإنه فشل في الكشف عن العجز المستمر في الفئران. توضح هذه الدراسة أن قياس السلوك مخلب-السحب هو تحليل أكثر حساسية للإصابة المخ في الفئران.

مخلب السحب: رواية، والتحليل الحساسة للماوس اختبار اسطوانة

The cylinder test is routinely used to predict focal ischemic damage to the forelimb motor cortex in rodents. When placed in the cylinder, rodents explore ...

paw-dragging-a-novel-sensitive-analysis-of-the-mouse-cylinder-test

Research

JoVE Journal

Behavior

Inchworming: A Novel Motor Stereotypy in the BTBR T+ Itpr3tf/J Mouse Model of Autism

Autism Spectrum Disorder (ASD) is a behaviorally defined neurodevelopmental disorder characterized by decreased reciprocal social interaction, abnormal communication, and repetitive behaviors with restricted interest. As diagnosis is based on clinical criteria, any potentially relevant rodent models of this heterogeneous disorder should ideally recapitulate these diverse behavioral traits. The BTBR T+ Itpr3tf/J (BTBR) mouse is an established animal model of ASD, displaying repetitive behaviors such as increased grooming, as well as cognitive inflexibility. With respect to social interaction and interest, the juvenile play test has been employed in multiple rodent models of ASD. Here, we show that when BTBR mice are tested in a juvenile social interaction enclosure containing sawdust bedding, they display a repetitive synchronous digging motion. This repetitive motor behavior, referred to as &#34;inchworming,&#34; was named because of the stereotypic nature of the movements exhibited by the mice while moving horizontally across the floor. Inchworming mice must use their fore- and hind-limbs in synchrony to displace the bedding, performing a minimum of one inward and one outward motion. Although both BTBR and C56BL/6J (B6) mice exhibit this behavior, BTBR mice demonstrate a significantly higher duration and frequency of inchworming and a decreased latency to initiate inchworming when placed in a bedded enclosure. We conclude that this newly described behavior provides a measure of a repetitive motor stereotypy that can be easily measured in animal models of ASD.

Inchworming: A Novel Motor Stereotypy in the BTBR T+ Itpr3tf/J Mouse Model of Autism

Inchworming is a highly repetitive synchronous digging motion displayed by BTBR T+ Itpr3tf/J (BTBR) mice when placed in a testing cage with sufficient sawdust bedding. The procedure is a modification of the juvenile social interaction protocol and is used here to assess repetitive motor stereotypies relevant to Autism Spectrum Disorder.

Inchworming: رواية موتور الرتابة في BTBR<em&gt; T<sup&gt; +</sup&gt; Itpr3<sup&gt; فريق العمل</sup</em&gt; / J نموذج الفأر من التوحد

Autism Spectrum Disorder (ASD) is a behaviorally defined neurodevelopmental disorder characterized by decreased reciprocal social interaction, abnormal ...

Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice

The contextual and cued fear conditioning test is one of the behavioral tests that assesses the ability of mice to learn and remember an association between environmental cues and aversive experiences. In this test, mice are placed into a conditioning chamber and are given parings of a conditioned stimulus (an auditory cue) and an aversive unconditioned stimulus (an electric footshock). After a delay time, the mice are exposed to the same conditioning chamber and a differently shaped chamber with presentation of the auditory cue. Freezing behavior during the test is measured as an index of fear memory. To analyze the behavior automatically, we have developed a video analyzing system using the ImageFZ application software program, which is available as a free download at http://www.mouse-phenotype.org/. Here, to show the details of our protocol, we demonstrate our procedure for the contextual and cued fear conditioning test in C57BL/6J mice using the ImageFZ system. In addition, we validated our protocol and the video analyzing system performance by comparing freezing time measured by the ImageFZ system or a photobeam-based computer measurement system with that scored by a human observer. As shown in our representative results, the data obtained by ImageFZ were similar to those analyzed by a human observer, indicating that the behavioral analysis using the ImageFZ system is highly reliable. The present movie article provides detailed information regarding the test procedures and will promote understanding of the experimental situation.

This article presents a protocol for a contextual and cued fear conditioning test using a video analyzing system to assess fear learning and memory in mice.

السياقية وملقن الخوف تكييف اختبار باستخدام نظام تحليل الفيديو في الفئران

The contextual and cued fear conditioning test is one of the behavioral tests that assesses the ability of mice to learn and remember an association ...

The Forced Swim Test as a Model of Depressive-like Behavior

The goal of the present protocol is to describe the forced swim test (FST), which is one of the most commonly used assays for the study of depressive-like behavior in rodents. The FST is based on the assumption that when placing an animal in a container filled with water, it will first make efforts to escape but eventually will exhibit immobility that may be considered to reflect a measure of behavioral despair. This test has been extensively used because it involves the exposure of the animals to stress, which was shown to have a role in the tendency for major depression. Additionally, the FST has been shown to share some of the factors that are influenced or altered by depression in humans, including changes in food consumption, sleep abnormalities and drug-withdrawal-induced anhedonia. The main advantages of this procedure are that it is relatively easy to perform and that its results are easily and quickly analyzed. Moreover, its sensitivity to a broad range of antidepressant drugs that makes it a suitable screening test is one of the most important features leading to its high predictive validity. Despite its appeal, this model has a number of disadvantages. First, the issue of chronic augmentation is problematic in this test because in real life patients need to be treated for at least several weeks before they experience any relief from their symptoms. Last, due to the aversiveness of the FST, it is important to take into account possible influences it might have on brain structure/function if brain analyses are to be carried out following this procedure.

This protocol describes the forced swim test, which is used for the study of depressive-like behavior in rodents. This procedure involves placing an animal in a container filled with water that eventually will lead to the exhibition of immobility behavior, which is considered to reflect behavioral despair.

والسباحة الاختبار القسري كنموذج للاكتئاب مثل السلوك

The goal of the present protocol is to describe the forced swim test (FST), which is one of the most commonly used assays for the study of depressive-like ...

Morris Water Maze Test: Optimization for Mouse Strain and Testing Environment

The Morris water maze (MWM) is a commonly used task to assess hippocampal-dependent spatial learning and memory in transgenic mouse models of disease, including neurocognitive disorders such as Alzheimer&#8217;s disease. However, the background strain of the mouse model used can have a substantial effect on the observed behavioral phenotype, with some strains exhibiting superior learning ability relative to others. To ensure differences between transgene negative and transgene positive mice can be detected, identification of a training procedure sensitive to the background strain is essential. Failure to tailor the MWM protocol to the background strain of the mouse model may lead to under- or over- training, thereby masking group differences in probe trials. Here, a MWM protocol tailored for use with the F1 FVB/N x 129S6 background is described. This is a frequently used background strain to study the age-dependent effects of mutant P301L tau (rTg(TauP301L)4510 mice) on the memory deficits associated with Alzheimer&#8217;s disease. Also described is a strategy to re-optimize, as dictated by the particular testing environment utilized.

This manuscript describes a Morris water maze (MWM) protocol tailored for use with a commonly used mouse model of Alzheimer's disease. The MWM is widely used in transgenic mouse models. Implementation of a procedure sensitive to the background strain of the mouse model is essential for detecting group differences.

موريس المياه متاهة الاختبار: الأمثل للماوس الانفعال والبيئة اختبار

 The Morris water maze (MWM) is a commonly used task to assess hippocampal-dependent spatial learning and memory in transgenic mouse models of disease, ...

A Novel Approach to Assess Motor Outcome of Deep Brain Stimulation Effects in the Hemiparkinsonian Rat: Staircase and Cylinder Test

Deep brain stimulation of the subthalamic nucleus is an effective treatment option for Parkinson&#39;s disease. In our lab we established a protocol to screen different neurostimulation patterns in hemiparkinsonian (unilateral lesioned) rats. It consists of creating a unilateral Parkinson&#39;s lesion by injecting 6-hydroxydopamine (6-OHDA) into the right medial forebrain bundle, implanting chronic stimulation electrodes into the subthalamic nucleus and evaluating motor outcomes at the end of 24 hr periods of cable-bound external neurostimulation. The stimulation was conducted with constant current stimulation. The amplitude was set 20% below the individual threshold for side effects. The motor outcome evaluation was done by the assessment of spontaneous paw use in the cylinder test according to Shallert and by the assessment of skilled reaching in the staircase test according to Montoya. This protocol describes in detail the training in the staircase box, the cylinder test, as well as the use of both in hemiparkinsonian rats. The use of both tests is necessary, because the staircase test seems to be more sensitive for fine motor skill impairment and exhibits greater sensitivity to change during neurostimulation. The combination of the unilateral Parkinson model and the two behavioral tests allows the assessment of different stimulation parameters in a standardized way.

Deep brain stimulation (DBS) is an effective treatment option for Parkinson's disease. We established a study design to screen novel stimulation paradigms in rats. The protocol describes the use of the staircase test and cylinder test for motor outcome assessment in DBS treated hemiparkinsonian rats.

أسلوب فريد لتقييم نتائج موتور من أعماق تحفيز الدماغ آثار في الجرذ Hemiparkinsonian: الدرج واختبار اسطوانة

Deep brain stimulation of the subthalamic nucleus is an effective treatment option for Parkinson&#39;s disease. In our lab we established a protocol to ...

The Treadmill Fatigue Test: A Simple, High-throughput Assay of Fatigue-like Behavior for the Mouse

Fatigue is a prominent symptom in many diseases and disorders and reduces quality of life for many people. The lack of clear pathogenesis and failure of current interventions to adequately treat fatigue in all patients leaves a need for new treatment options. Despite the therapeutic need and importance of preclinical research in helping identify promising novel treatments, few preclinical assays of fatigue are available. Moreover, the most common preclinical assay used to assess fatigue-like behavior, voluntary wheel running, is not suitable for use with some strains of mice, may not be sensitive to drugs that reduce fatigue, and has relatively low throughput. The current protocol describes a novel, non-voluntary preclinical assay of fatigue-like behavior, the treadmill fatigue test, and provides evidence of its efficacy in detecting fatigue-like behavior in mice treated with a chemotherapy drug known to cause fatigue in humans and fatigue-like behavior in animals. This assay may be a beneficial alternative to wheel running, as fatigue-like behavior and potential interventions can be assessed in a greater number of mice over a shorter time frame, thus permitting faster discovery of new therapeutic options.

Fatigue is a common, undertreated and frequently poorly-understood symptom in many diseases and disorders. New preclinical assays of fatigue may help to improve current understanding and future treatment of fatigue. To that end, the current protocol provides a novel means of measuring fatigue-like behavior in the mouse.

المطحنة التعب الاختبار: مقايسة بسيطة، عالية الإنتاجية من التعب مثل سلوك للماوس

Fatigue is a prominent symptom in many diseases and disorders and reduces quality of life for many people. The lack of clear pathogenesis and failure of ...

The 4 Mountains Test: A Short Test of Spatial Memory with High Sensitivity for the Diagnosis of Pre-dementia Alzheimer's Disease

This protocol describes the administration of the 4 Mountains Test (4MT), a short test of spatial memory, in which memory for the topographical layout of four mountains within a computer-generated landscape is tested using a delayed match-to-sample paradigm. Allocentric spatial memory is assessed by altering the viewpoint, colors and textures between the initially presented and target images.
Allocentric spatial memory is a key function of the hippocampus, one of the earliest brain regions to be affected in Alzheimer&#39;s disease (AD) and impairment of hippocampal function predates the onset of dementia. It was hypothesized that performance on the 4MT would aid the diagnosis of predementia AD, which manifests clinically as Mild Cognitive Impairment (MCI).
The 4MT was applied to patients with MCI, stratified further based on cerebrospinal fluid (CSF) AD biomarker status (10 MCI biomarker positive, 9 MCI biomarker negative), and with mild AD dementia, as well as healthy controls. Comparator tests included tests of episodic memory and attention widely accepted as sensitive measures of early AD. Behavioral data were correlated with quantitative MRI measures of the hippocampus, precuneus and posterior cingulate gyrus.
4MT scores were significantly different between the two MCI groups (p = 0.001), with a test score of &#8804;8/15 associated with 100% sensitivity and 78% specificity for the classification of MCI with positive AD biomarkers, i.e., predementia AD. 4MT test scores correlated with hippocampal volume (r = 0.42) and cortical thickness of the precuneus (r = 0.55).
In conclusion, the 4MT is effective in identifying the early stages of AD. The short duration, easy application and scoring, and favorable psychometric properties of the 4MT fulfil the need for a simple but accurate diagnostic test for predementia AD.

The 4 Mountains Test: A Short Test of Spatial Memory with High Sensitivity for the Diagnosis of Pre-dementia Alzheimer&#039;s Disease

This article describes the 4 Mountains Test (4MT), a hippocampus-dependent test of working allocentric spatial memory. The hippocampus is affected early in Alzheimer's disease (AD) and this article outlines the 4MT methodology and results of patient testing, which demonstrates the value of the 4MT in the diagnosis of pre-dementia AD.

4 جبال الاختبار: اختبار قصير من الذاكرة المكانية مع حساسية عالية لتشخيص ما قبل الخرف مرض الزهايمر

This protocol describes the administration of the 4 Mountains Test (4MT), a short test of spatial memory, in which memory for the topographical layout of ...

A Method to Test the Effect of Environmental Cues on Mating Behavior in Drosophila melanogaster

An individual's sexual drive is influenced by genotype, experience and environmental conditions. How these factors interact to modulate sexual behaviors remains poorly understood. In Drosophila melanogaster, environmental cues, such as food availability, affect mating activity offering a tractable system to investigate the mechanisms modulating sexual behavior. In D. melanogaster, environmental cues are often sensed via the chemosensory gustatory and olfactory systems. Here, we present a method to test the effect of environmental chemical cues on mating behavior. The assay consists of a small mating arena containing food medium and a mating couple. The mating frequency for each couple is continuously monitored for 24 h. Here we present the applicability of this assay to test environmental compounds from an external source through a pressurized air system as well as manipulation of the environmental components directly in the mating arena. The use of a pressurized air system is especially useful to test the effect of very volatile compounds, while manipulating components directly in the mating arena can be of value to ascertain a compound's presence. This assay can be adapted to answer questions about the influence of genetic and environmental cues on mating behavior and fecundity as well as other male and female reproductive behaviors.

A Method to Test the Effect of Environmental Cues on Mating Behavior in Drosophila melanogaster

We demonstrate an assay to analyze the environmental and genetic cues that influence mating behavior in the fruit fly Drosophila melanogaster.

وهناك طريقة لاختبار تأثير الإشارات البيئية على سلوك التزاوج في<em&gt; ذبابة الفاكهة ميلانوغاستر</em

An individual's sexual drive is influenced by genotype, experience and environmental conditions. How these factors interact to modulate sexual behaviors ...

Novel Object Recognition Test for the Investigation of Learning and Memory in Mice

The object recognition test (ORT) is a commonly used behavioral assay for the investigation of various aspects of learning and memory in mice. The ORT is fairly simple and can be completed over 3 days: habituation day, training day, and testing day. During training, the mouse is allowed to explore 2 identical objects. On test day, one of the training objects is replaced with a novel object. Because mice have an innate preference for novelty, if the mouse recognizes the familiar object, it will spend most of its time at the novel object. Due to this innate preference, there is no need for positive or negative reinforcement or long training schedules. Additionally, the ORT can also be modified for numerous applications. The retention interval can be shortened to examine short-term memory, or lengthened to probe long-term memory. Pharmacological intervention can be used at various times prior to training, after training, or prior to recall to investigate different phases of learning (i.e., acquisition, early or late consolidation, or recall). Overall, the ORT is a relatively low-stress, efficient test for memory in mice, and is appropriate for the detection of neuropsychological changes following pharmacological, biological, or genetic manipulations.

The object recognition test (ORT) is a simple and efficient assay for evaluating learning and memory in mice. The methodology is described below.

اختبار كائن رواية الاعتراف للتحقيق في التعلم والذاكرة في الفئران

The object recognition test (ORT) is a commonly used behavioral assay for the investigation of various aspects of learning and memory in mice. The ORT is ...

Measuring Active and Passive Tameness Separately in Mice

Domesticated animals such as dogs and laboratory mice show a high level of tameness, which is important for humans to handle them easily. Tameness has two behavioral components: a reluctance to avoid humans (passive tameness) and a motivation to approach humans (active tameness). To quantify these components in mice, we previously developed behavioral tests for active tameness, passive tameness, and the willingness to stay on a human hand, each designed to be completed within 3 min. The data obtained were used for selective breeding, with a large number of mice analyzed per generation. The active tameness test measures the movement of the mouse toward a human hand and the contact it engages in. The passive tameness test measures the duration of time that a mouse tolerates human touch. In the stay-on-hand test, a mouse is placed on a human hand and touched slowly using the thumb of that hand; the duration of time that the animal remains on the hand is measured. Here, we describe the test set-up and apparatus, explain the procedures, and discuss the appropriate data analysis. Finally, we explain how to interpret the results.

Tameness in animals includes a reduction of avoidance responses to humans (passive tameness) and an increase in active approaches to humans (active tameness). Here, we describe detailed protocols for three behavioral tests (active tameness, passive tameness, and stay-on-hand tests) to separately measure the active and passive tameness of mice.

قياس تامينيس الإيجابي والسلبي بشكل منفصل في الفئران

Domesticated animals such as dogs and laboratory mice show a high level of tameness, which is important for humans to handle them easily. Tameness has two ...