Name: paw-dragging: a novel, sensitive analysis of the mouse cylinder test
Uploaded: 2015-04-29T16:00:00
Description: Watch this Scientific Journal Video about Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test at JoVE.com

我们感谢约翰·韦尔先生和特里Upshall先生为他们的技术专长和援助的摄影和录像。这项工作是通过操作补助JLV从健康研究的加拿大学院和纽芬兰的研究与发展公司和心脏病及中风的加拿大加拿大合作基金会中风回收催化剂赠款的支持。 RBR是基思·格里菲斯纪念心脏及中风基金会研究生奖学金的获得者。

伦理声明：所有实验由纽芬兰的动物保健伦理委员会纪念大学根据加拿大议会关于动物饲养的指导方针批准。 1.小鼠<ol><li>用成年鼠。在这项研究中，成年雄性FVBN小鼠（n = 10）之间2-4个月的被使用。家鼠在12:12小时反向明暗周期，并提供标准啮齿动物食物和水自由采食 。 </li></ol> 2.材料所需圆柱测试<ol><li>获得一台具有透明的顶部拍摄的气瓶检验。表的尺寸是无关...

<ol>
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量化爪拖动行为时，在气缸测试有以下要点建立：我）量化数PAW-拖动与总爪子触摸脑损伤之前，每个爪子建立一个基线;二）量化爪拖动与总爪子触摸每个爪子下面的缺血性损伤的数量;和iii）一个爪拖动并沿气缸壁的爪子的期间小鼠的躯干的横向转动的横向运动区分。 PAW-拖动是出现损伤后的前肢感觉运动皮层的新型行为。的爪式拖行为的外观因此可以用作该前肢感觉皮层已...

神经再生战略的目标是展示两个组织修复和功能的恢复。功能恢复通常评估与测量功能缺陷，在这种情况下，涉及了与损害特定脑区域相关联的运动技能行为测试。创伤性脑损伤或脑皮层的感觉运动前肢区局部缺血损伤可以由许多行为测试来证明。一个这样的试验中，在气缸试验被广泛使用的大鼠，以评估在前肢活动1的功能缺陷。测试具有低的建立费用，仅需要一个气缸，照相机和表用透明顶部。这是很容易，因为它是基于啮齿动物的自然探索行为来管理，所以训练前和食物剥夺或奖励不是必需的。尽管有这些众多的优势，在气缸测试下，利用下面的焦点受伤的前肢s到评估小鼠前肢赤字ensorimotor皮层，这是我们归因于对鼠标行为的气瓶检验分析。前肢不对称是经典测度分析气缸测试。当放置在气缸中，啮齿动物，自然由饲养到其后肢和接触汽缸壁与他们的前肢的爪子对姿势平衡探索汽缸的壁。爪接触与壁与每个前肢的数目很容易被这种探索气缸期间拍摄啮齿类定量。当受影响的前肢的爪子，使更少的接触与墙体比未受影响前肢的爪子，表明损坏对侧感觉皮层发生前肢不对称。在大鼠中，血管收缩剂的内部皮质注射剂，内皮素（ET-1），进前肢感觉皮层引起局灶性缺血损伤，这导致行为缺陷在对侧前肢。在对侧前肢使用赤字很容易检测到的变化f中在大鼠1-3缸测试orelimb不对称。在对比然而大鼠，改变前肢不对称是可变的，在小鼠中以下可比的ET-1注射4-6不太一致。在这里，我们展示了一个新的汽缸测试前肢行为分析 - 中爪拖行为分析。我们以前曾表明，爪拖动分析是损坏的小鼠比经典前肢不对称分析前肢感觉皮层的更灵敏的测量，因此是适用于各种局灶性皮质损伤模型。 检查如何前爪接触汽缸壁以下的前肢感觉皮层缺血损伤揭示了一种新的行为在小鼠-爪拖动4。当鼠标站在其后腿探索气缸壁，然后沿筒​​壁对其中线或向下的墙面上，同时拖动其受影响（禁忌病变）发生足一爪子拖它的前爪不受影响提供靠墙的姿势支持。 PAW-拖动很少发生在未受伤的老鼠因此爪子拖动的外观是受伤的前肢感觉运动皮层4正面指标。我们先前量化以下的前肢感觉皮层ET-1的局部缺血损伤的小鼠爪拖动行为和表现出持续爪拖动行为小鼠长达两周中风后4。在这里，我们表明，爪拖动行为是持续长达四周后中风。爪的拖行为分析提供了一种新的和敏感的工具，以评估在小鼠前肢感觉运动皮层局灶性缺血性脑损伤。以其物美价廉的设置，便于管理和得分使这个简单，但非常有用的工具，以快速评估在小鼠前肢行为缺陷。

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>Plexi-glass cylinder</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>17.5cm high, 9.5cm outer diameter, 8.8cm inner diameter, wall thickness 0.35cm (or 3.5 mm)</td>
		</tr>
		<tr>
			<td>viewing table</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>54x56x66.5cm (width x length x height), top of table is a 51x51cm sheet of plexiglass.</td>
		</tr>
		<tr>
			<td>mirror</td>
			<td>N/A</td>
			<td>N/A</td>
			<td>34x58cm mirror</td>
		</tr>
		<tr>
			<td>video camera</td>
			<td>Sony</td>
			<td>DCR-SR42</td>
			<td>Video camera with onboard storage, SD functionality, 40x optical zoom</td>
		</tr>
		<tr>
			<td>computer</td>
			<td>Dell</td>
			<td>Optiplex 760</td>
			<td>Processor: Intel, 3.0 GHz, Memory 4.00GB (RAM)&#160;</td>
		</tr>
		<tr>
			<td>computer monitor</td>
			<td>Samsung</td>
			<td>S22C350H</td>
			<td></td>
		</tr>
		<tr>
			<td>Excel (Microsoft Office Professional Plus)</td>
			<td>Microsoft</td>
			<td>v14.0.7106.5003</td>
			<td></td>
		</tr>
		<tr>
			<td>VLC Media Player</td>
			<td>Video LAN</td>
			<td>v2.1.2</td>
			<td>Media player with playback speed modulation and video support</td>
		</tr>
		<tr>
			<td>External Hard Drive</td>
			<td>Western Digital</td>
			<td>WDBAAU0020HBK-01</td>
			<td>2 TB</td>
		</tr>
	</tbody>
</table>

paw-dragging: a novel, sensitive analysis of the mouse cylinder test

气缸试验常规地用于预测给前肢运动皮层在啮齿类动物局灶性缺血损伤。当放置在气缸中，啮齿动物探索由饲养和触摸气缸的壁与他们的前肢的爪子对姿势支承。缺血性损伤的前肢感觉运动皮层，老鼠更多地依赖于他们的前肢不受影响的爪子导致其受影响的爪子被称为前肢不对称较少触及姿势的支持。与此相反，在小鼠脑局灶性缺血损伤未能导致前肢不对称可比一致赤字。虽然前肢不对称赤字很少观察到，老鼠也表现出了一种新的行为中风后称为“爪拖”。 PAW-拖动是鼠标沿筒壁，而不是直接从墙壁从后方拆卸到一个四条腿的立场时，推下拖动其受影响的爪子的倾向。之前我们已经表明，PAW-拖动行为是小皮质缺血性损伤的前肢运动皮层高度敏感。在这里，我们为爪拖分析了详细的方案。我们定义什么是爪子拖就是和演示如何量化爪拖行为。气缸试验是一种简单且廉价的检验管理，并且不需要预训练或食物剥夺策略。在使用爪拖动分析与汽缸测试，它填补利基预测皮质缺血性损伤如photothrombosis和内皮素-1（ET-1）诱导的缺血 - 两个模型被不断增加的流行度，并产生更小的焦距受伤比大脑中动脉闭塞。最后，测量气缸测试爪拖动行为将使功能恢复使用的转基因小鼠品系，其中前面的前肢不对称分析未能检测到一致的赤字广泛队列皮质损伤后的研究。

之前我们已经证实看来，爪子拖行为遵循局灶性缺血损伤的前肢感觉运动皮层和损坏4的正面指标。 ET-1的内皮层注射入前肢感觉皮层被用来诱导局部缺血损伤（ 图8A，B）。本研究的爪子拖动行为是否延期超过14天受伤后其潜在用途，以评估功能恢复。小鼠在当日气缸试验前的ET-1注射的手术前的时间点，并在天1，3，7，14，21和28后的损伤进行测试。在每个时间点，爪子触摸和?...

Watch this Scientific Journal Video about Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test at JoVE.com

Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test

气缸测试的经典前肢不对称分析常规地用于评估脑损伤或中风大鼠行为缺陷;但是，它无法检测一致赤字小鼠。这项研究表明，量化爪拖动行为是脑损伤的小鼠更敏感的分析。

PAW-拖动：一本小说，鼠标缸试验敏感性分析

The cylinder test is routinely used to predict focal ischemic damage to the forelimb motor cortex in rodents. When placed in the cylinder, rodents explore ...

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