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9.8K Views
•
11:24 min
March 25th, 2015
DOI :
10.3791/52814-v
Chapters
0:05
Title
1:23
Cloning Emerald GFP into an Expression Vector Containing Two-finger ZiF Domains
4:22
Purification of the ZiF-EmGFP Fusion Protein
7:17
Protein Transduction of HeLa Cells
10:07
Results: Fusion of 2F-ZiF Domains to EmGFP Enables Delivery of EmGFP to HeLa Cells
10:57
Conclusion
Summary
锌指结构域是本质细胞渗透性和能够介导蛋白输送到广泛的哺乳动物细胞类型的。这里,一个详细的一步一步的协议用于实现锌指技术为细胞内蛋白输送呈现。
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