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11.9K Views
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15:57 min
July 11th, 2015
DOI :
10.3791/52833-v
Chapters
0:00
Title
1:40
Methods
2:05
Long-term Culture
3:38
Elispot Assay Day 1
4:30
Elispot Assay Day 2
5:15
Adherent Cell Isolation
5:37
Flow Cytometry Plate Preparation
6:15
Harvest the 13 Day Long-term Cells
9:05
Elispot Assay Day 3
10:04
Alkaline Phosphatase Preparation
11:08
Substrate Solution Preparation
11:55
Reading the Plate
12:12
Performing Flow Cytometry
12:25
Results
14:37
Conclusions
Summary
长期培养的干扰素γ酶联免疫斑点法被用作中央记忆应答的量度并且与保护性抗分枝杆菌疫苗的反应。用该测定中,外周血单核细胞刺激与分枝杆菌抗原和IL-2 14天,使分化和扩展中央记忆T细胞。
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