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11.9K Views
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15:57 min
July 11th, 2015
DOI :
10.3791/52833-v
Chapters
0:00
Title
1:40
Methods
2:05
Long-term Culture
3:38
Elispot Assay Day 1
4:30
Elispot Assay Day 2
5:15
Adherent Cell Isolation
5:37
Flow Cytometry Plate Preparation
6:15
Harvest the 13 Day Long-term Cells
9:05
Elispot Assay Day 3
10:04
Alkaline Phosphatase Preparation
11:08
Substrate Solution Preparation
11:55
Reading the Plate
12:12
Performing Flow Cytometry
12:25
Results
14:37
Conclusions
Summary
長期培養インターフェロンγ酵素結合免疫アッセイは、中央メモリー応答の尺度として用い、保護抗マイコバクテリアワクチン応答と相関されます。このアッセイでは、末梢血単核細胞を、分化、中央メモリーT細胞の拡大を可能にする、14日間マイコバクテリア抗原およびインターロイキン2で刺激します。
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