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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

10.8K Views

12:19 min

June 27th, 2015

June 27th, 2015

10,812 Views

0:05

Title

2:35

Kidney Flush

4:25

Isolation of the Rat Glomeruli

5:35

Single-channel Patch-clamp Electrophysiology

6:30

Ratiometric Confocal Fluorescence Measurements of Intracellular Calcium Concentration in the Podocytes

8:08

Image Analysis for the Calcium Measurements

9:46

Results: Recording of Single Ion Channels Activity in the Podocytes of Freshly Isolated Glomeruli and Real-time Detection of Calcium Concentration Changes

11:16

Conclusion

Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.

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