Coupling of LBD Proteins to AminoLink Coupling Resin
4:11
Blocking Remaining Sites
5:43
Purification of Nurr1 Specific Antibodies
7:21
ELISA-based Analysis of Purified Anti-Nurr1 Antibody
10:04
Results: Pre-adsorption Against Cross-reactive Antigens Increases Specificity of Anti-Nurr1 Polyclonal Antibodies
11:56
Conclusion
Here, we selectively target antibodies against a specific member of a highly conserved family of proteins by immunizing animals with their most divergent regions followed by removing cross reactive antibodies by pre-adsorption.