Name: in vitro modeling of cancerous neural invasion: the dorsal root ganglion model
Uploaded: 2016-04-12T14:00:00
Description: Watch this Scientific Journal Video about In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model at JoVE.com

Edith Suss-Toby is thanked for her assistance in the time-lapse microscopy and image analysis. Nofar Rada is thanked for the artistic work.

6 주 된 여성 C57BL / CJ 마우스에 네살 (할란은, 예루살렘, 이스라엘) 평가 및 실험 동물 관리 사양의 인증을위한 협회에 따르면 실험에 사용 하였다. 모든 실험 절차는 기관 동물 관리 및 사용위원회 농업 규정의 부에 따라 수행 하였다. 1. 수확 척수 <ol><li> 공동이 챔버를 사용하여 마우스를 안락사. 이 전단력에 의한 신경절 뿌리에 손상을 줄 수 있습니다로 자궁 전위?...

<ol>
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이 문서에서는 신경 틈새에서 암의 미세 환경의 DRG 모델을 되풀이되었습니다 체외 모델을 제시한다. 비디오는 같은 마우스에서 DRG, 그 추출 및 ECM의 마지막으로, 그 배양으로 해부학 적 랜드 마크를 인식에서 시작하여 모든 단계를 보여줍니다. 공동 배양 암세포 나란히 DRG 또한 제공된다. 체외에서 신경초 틈새 미세 환경 연구를위한이 모델은 필수적 만드는 문헌에 기재된 체외</e...

직접 침윤, 림프 확산 및 hematogenic 확산 : 고체 종양은 세 가지 방법으로 전파. 그러나, 자주 신경을 따라, 보급을 무시 암 확산의 제 4 수단이있다. 암성 신경 침윤 (CNI)는 특히 두경부 1 전립선 2, 3 및 췌장 암의 암 확산의 공지의 경로이다. 4-8 CNI 선도 췌장 선암 개인의 80 % 이상에서 발생 복강 신경절 신경을 통해 확산 복막 종양. 이러한 neurotropic 암 세포는 중추 신경계 (CNS) 방향으로 신경을 따라 일방향으로 이주 할 수있는 독특한 능력을 가지고있다. (9)이 결과는 신경초 미세이 악성 성장을 지원하는 인자를 제공하는, 암 세포에 의해 이용 될 수 있다는 것을 시사한다. CNI 연구를위한 몇 체외 모델 중 하나는 후근 신경절 (DRG) / 암 세포 모델입니다. 이 모드엘 자주 갓 해리 배양 DRG의 준비에 인접한 세포 외 기질 (ECM)에서 재배되는이 모델, 마우스 또는 인간의 암 세포 라인에 신경 기질 암 세포 사이. 10-18 분비의 상호 작용을 연구하는 데 사용됩니다. 이 비디오 기사는 췌장 도관 선암에서 체외 CNI의 응용 프로그램을 보여줍니다.

<table border="0" cellpadding="0" cellspacing="0" width="710">
	<tbody>
		<tr height="20">
			<td height="20" style="height:20px;width:417px;">Equipments:</td>
			<td style="width:121px;"></td>
			<td style="width:108px;"></td>
			<td style="width:64px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Operating microscope</td>
			<td>Leica</td>
			<td>M205</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tiime Lapse System</td>
			<td>Zeiss</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Forceps</td>
			<td>Sigma-Aldrich</td>
			<td>F4142&#160;</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Surgical blade</td>
			<td>Sigma-Aldrich</td>
			<td>Z309036</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Scissors</td>
			<td>Sigma-Aldrich</td>
			<td>S3271</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">35mm petri dishes, glass bottom</td>
			<td>de groot</td>
			<td>60-627860</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Name</td>
			<td>Company&#160;</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Materials:</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">70% ethanol</td>
			<td>sigma</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Cold PBS</td>
			<td>Biological industries</td>
			<td>02-023-1A</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">DMEM</td>
			<td>Biological industries</td>
			<td>01-055-1A</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">FCS</td>
			<td>Rhenium</td>
			<td align="right">10108165</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Penicillin and streptomycin</td>
			<td>Biological industries</td>
			<td>01-031-1B</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sodium Pyruvate</td>
			<td>Biological industries</td>
			<td>03-042-1B</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">L-Glutamine</td>
			<td>Biological industries</td>
			<td>03-020-1B</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Growth factor depleted matrigel</td>
			<td>Trevigen</td>
			<td>3433-005-01</td>
			<td></td>
		</tr>
	</tbody>
</table>

in vitro modeling of cancerous neural invasion: the dorsal root ganglion model

One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These neurotropic cancer cells have a unique ability to migrate unidirectionally along nerves towards the central nervous system (CNS). The dorsal root ganglia (DRG)/cancer cell model is a three dimensional (3D) in vitro model frequently used for studying the interaction between neural stroma and cancer cells. In this model, mouse or human cancer cell lines are grown in ECM adjacent to preparations of freshly dissociated cultured DRG. In this article, the DRG isolation protocol from mice, and implantation in petri dishes for co-culturing with pancreatic cancer cells are demonstrated. Five days after implantation, the cancer cells made contact with the DRG neurites. Later, these cells formed bridgeheads to facilitate more extensive polarized, neurotropic migration of cancer cells.

<html> 비디오 현미경 영상을 사용하여, DRG는 암세포가 DRG에 대한 그들의 콜로니로부터 멀리 이주 동안 주입 후 신경 돌기 5-7 일간 발아 알 수있다. 이식 후 7 일째에 의해, 암 세포는 신경 돌기 (도 2)와 접촉. 이 프로토콜에서 사용되는 췌장암 세포의 전방 이동 지수는 다른 세포주 (QLL2, B16F) (도 3a)보다...

Watch this Scientific Journal Video about In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model at JoVE.com

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

This video article shows the use of the dorsal root ganglia (DRG)/cancer cell model in pancreatic ductal adenocarcinoma.

등쪽 뿌리 신경절 모델 : 암성 신경 침략의 생체 외 모델링에서

One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These ...

Research

JoVE Journal

Neuroscience

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

스프 레딩 우울증을 사용하여 에피소드와 만성 두통의 신경 면역 신호를 모델링 체외에서</em

Migraine and its transformation to chronic migraine are healthcare burdens in need of improved treatment options.  We seek to define how neural immune ...

연구

신경과학

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

교양 성인 지느러미 루트 신경절의 신경 세포와 축삭 재생의 유전 연구

It is well known that mature neurons in the central nervous system (CNS) cannot regenerate their axons after injuries due to diminished intrinsic ability ...

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

루트 신경절 신경 세포와 차별화 된 지방 유래 줄기 세포를 이룬다 :<em&gt; 체외</em&gt; 공동 문화 모델은 주변 신경 재생을 연구하는

Dorsal root ganglia (DRG) neurons, located in the intervertebral foramina of the spinal column, can be used to create an in vitro system facilitating the ...

An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

접근 방법은 등쪽 뿌리 신경절 뉴런의 정렬 및 수초를 강화하는

Axon regeneration is a chaotic process due largely to unorganized axon alignment. Therefore, in order for a sufficient number of regenerated axons to ...

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

등쪽 뿌리 신경절 사출 및 지느러미 루트 감각 축삭의 재생을위한 모델로 부상 크러시

Achieving axon regeneration after nervous system injury is a challenging task. As different parts of the central nervous system (CNS) differ from each ...

Laminotomy for Lumbar Dorsal Root Ganglion Access and Injection in Swine

Laminotomy 허리 지 루트 신경 절의 액세스 및 돼지에 주입

Dorsal root ganglia (DRG) are anatomically well defined structures that contain all primary sensory neurons below the head. This fact makes DRG attractive ...

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

단일 좌 골 신경 및 Cytometry 사용 하 여 단일 마우스에서 지 루트 신경 절에 면역 세포의 분석

Nerve-resident immune cells in the peripheral nervous system (PNS) are essential to maintaining neuronal integrity in a healthy nerve. The immune cells of ...

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

성인 마우스 지 루트 신경 절의 Electroporation Vivo에서 조사 포유류 축 삭 재생:

Electroporation is an essential non-viral gene transfection approach to introduce DNA plasmids or small RNA molecules into cells. A sensory neuron in the ...

Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure

전도 실패의 메커니즘을 검사하기 위해 연결된 Sciatic 신경을 가진 생체 내 단일 섬유 기록 및 손상되지 않은 등철 루트 신경절의 사용

Single-fiber recording has been a classical and effective electrophysiological technique over the last few decades because of its specific application for ...

Rapid Isolation of Dorsal Root Ganglion Macrophages

등갈 뿌리 신경절 대식세포의 신속한 절연

There are growing interests to study the molecular and cellular interactions among immune cells and sensory neurons in the dorsal root ganglia after ...