Name: acrylic resin molding based head fixation technique in rodents
Uploaded: 2016-01-12T13:00:00
Description: Watch this Scientific Journal Video about Acrylic Resin Molding Based Head Fixation Technique in Rodents at JoVE.com

This research was supported by Basic Science Research Program through the NRF funded by the Ministry of Education, Science and Technology (2010-0022362) and (2012R1A2A2A02046812).

伦理声明： 这个实验被批准为庆北大学实验动物管理和使用委员会，并根据指南实验动物的护理和使用（美国国立卫生研究院，1996年）进行。实验结束后，动物在二氧化碳安乐死牺牲。 在头固定杆的大鼠1.设计注：此步骤是一个一般原则的固定杆的设计。关于尺寸和材料的任何修改，可以根据动物或植入物。设计使用CAD软件头 - 固定杆?...

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This protocol demonstrates a simple and convenient head fixation method using materials at hand. For physically successful head fixation, the first critical steps during surgery is to rinse and dry the skull surface clearly before applying the dental adhesive resin cement. Second, this method does not uses screws implanting under temporal muscles and only depends on the screws on the skull, their perpendicular implantation against the surface of skull rim is crucial10. This reduces the possibility of accidenta...

头部固定或头部限制是通过在头骨的刚性夹紧附连框架或板固定动物的头的技术。该技术已通过精确的感官刺激，行为检测有或无神经记录清醒动物1-3和在睡眠-觉醒周期4 juxtacellular标签。它也可用于在体内脑成像5,6。现在，头部固定技术在神经科学和行为学研究中常用的工具。 基本上，传统的头部固定要求的金属头桩头骨6-8之上手术附件。附件随后夹紧到一固定平台，从而在头部的固定化。神经生理学研究在清醒的动物，如神经和/或光学记录，脑电路的干预，脑成像是伴随着使用牙科丙烯酸植入手术。因此，它似乎实现了头部固定在这些实验室的时候是实验需要一个简单的任务。帧应该被设计成重量轻和小到不会打扰在他们的家笼动物的自然行为。它也提供了机械刚性时夹紧在固定的平台。它进一步具有生物相容性取决于实验目的。因此，在技术上难以设计和制造在一般实验室设备，例如头职位。 在这项研究中，一种新的头部固定方法进行说明，提供了通过方便夹紧机械刚度，而无需使用金属头的职位。通过使用该方法，头部固定可以一般实验室条件下，其中，通常进行在啮齿类动物脑研究植入手术下进行。金属棒头桩被替换为丙烯酸树脂水泥由一个特制的头固定杆和模制工艺模制部分IMPLAN期间完成塔季翁手术。该固定杆提供了与其他植入物，如神经录像探头，刺激电极高空间的兼容性。还可以简化步骤通过除去螺钉紧固夹紧动物的头在固定平台上的。我们使用这种方法，在以前的行为研究和我们的结论是，这种方法很容易实现和方便，在实际的实验9。

<table border="0" cellpadding="0" cellspacing="0" width="901">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Dental acrylic resin</td>
			<td style="width:199px;">Lang Dental Manufacturing</td>
			<td style="width:129px;">Ortho-Jet</td>
			<td style="width:343px;">to make molded mounter</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Paraffin film</td>
			<td style="width:199px;">Bemis</td>
			<td style="width:129px;">Parafilm</td>
			<td>to prevent adhesion of acrylic resin to the bar</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Dental adhesive</td>
			<td style="width:199px;">Sun Medical</td>
			<td style="width:129px;">Super-Bond C&#38;B</td>
			<td>to bond between acrylic resin and skull</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Plexglass or other acrylic plate</td>
			<td style="width:199px;"></td>
			<td style="width:129px;"></td>
			<td>to make fixation bar and box platform</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:231px;">Cyanoacrylate</td>
			<td style="width:199px;"></td>
			<td style="width:129px;"></td>
			<td>to bond acrylic plates</td>
		</tr>
	</tbody>
</table>

acrylic resin molding based head fixation technique in rodents

Head fixation is a technique of immobilizing animal's head by attaching a head-post on the skull for rigid clamping. Traditional head fixation requires surgical attachment of metallic frames on the skull. The attached frames are then clamped to a stationary platform resulting in immobilization of the head. However, metallic frames for head fixation have been technically difficult to design and implement in general laboratory environment. In this study, we provide a novel head fixation method. Using a custom-made head fixation bar, head mounter is constructed during implantation surgery. After the application of acrylic resin for affixing implants such as electrodes and cannula on the skull, additional resins applied on top of that to build a mold matching to the port of the fixation bar. The molded head mounter serves as a guide rails, investigators conveniently fixate the animal&#8217;s head by inserting the head mounter into the port of the fixation bar. This method could be easily applicable if implantation surgery using dental acrylics is necessary and might be useful for laboratories that cannot easily fabricate CNC machined metal head-posts.

此头固定方法适用于大鼠和小鼠。共有8个大鼠接受刺激电极插入内侧前脑束的双侧植入。对于四脱离出来的，定制的钨丝电极（微型硬盘）被加注入到M1前肢区域（图2C）。对小鼠（n = 2），四不锈钢线电极注入到额叶/枕叶皮质区。在手术过程中，头贴片机进行成型的头部固定在大鼠和小鼠，作为描述协议第2节从手术中恢复后，他们被暴露在头部固定的或者习惯或行为的任务日常会?...

Watch this Scientific Journal Video about Acrylic Resin Molding Based Head Fixation Technique in Rodents at JoVE.com

Acrylic Resin Molding Based Head Fixation Technique in Rodents

Traditional head fixation techniques have used metallic frames attached on the skull as an interface for head fixation apparatus. This protocol demonstrates a frameless, acrylic resin molding based head fixation technique for behavioral tasks in rodents.

在鼠害丙烯酸树脂模塑基头固定技术

Head fixation is a technique of immobilizing animal's head by attaching a head-post on the skull for rigid clamping. Traditional head fixation requires ...

Research

JoVE Journal

Neuroscience

Whole Animal Perfusion Fixation for Rodents

整体动物灌注固定鼠害

The goal of fixation is to rapidly and uniformly preserve tissue in a life-like state. While placing tissue directly in fixative works well for small ...

科研

神经科学

A Low Cost Setup for Behavioral Audiometry in Rodents

在啮齿动物的行为测听的低成本设置

In auditory animal research it is crucial to have precise information about basic hearing parameters of the animal subjects that are involved in the ...

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

运行轮活动在啮齿类动物的昼夜节律的记录和分析

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

嗅鞘细胞移植周围神经损伤后，以评估功能恢复

Olfactory ensheathing cells (OECs) are neural crest cells which allow growth and regrowth of the primary olfactory neurons. Indeed, the primary olfactory ...

Deep Brain Stimulation with Simultaneous fMRI in Rodents

深部脑刺激与同步功能磁共振成像在鼠害

In order to visualize the global and downstream neuronal responses to deep brain stimulation (DBS) at various targets, we have developed a protocol for ...

Transcranial Electrical Brain Stimulation in Alert Rodents

警惕啮齿动物的经颅电脑刺激

Transcranial electrical brain stimulation can modulate cortical excitability and plasticity in humans and rodents. The most common form of stimulation in ...

Intracranial Pharmacotherapy and Pain Assays in Rodents

颅内药物治疗和啮齿动物疼痛检测

Pain is a salient sensory experience with affective and cognitive dimensions. However, central mechanisms for pain remain poorly understood, hindering the ...

The Power of Interstimulus Interval for the Assessment of Temporal Processing in Rodents

间刺激间隔对啮齿类动物时间处理评估的力量

Temporal processing deficits have been implicated as a potential elemental dimension of higher-level cognitive processes, commonly observed in ...

A Revised Surgical Approach to Induce Endolymphatic Hydrops in the Guinea Pig

几内亚猪诱导内部水解的修正手术方法

Endolymphatic hydrops is an enlargement of scala media that is most often associated with Meniere&#39;s disease, though the pathophysiologic mechanism(s) ...

Low-intensity Blast Wave Model for Preclinical Assessment of Closed-head Mild Traumatic Brain Injury in Rodents

低强度冲击波模型用于啮齿动物闭头轻度创伤性脑损伤的临床前评估

Traumatic brain injury (TBI) is a large-scale public health problem. Mild TBI is the most prevalent form of neurotrauma and accounts for a large number of ...