Name: chronic constriction injury of the rat's infraorbital nerve (ion-cci) to study trigeminal neuropathic pain
Uploaded: 2015-09-21T15:45:00
Description: Watch this Scientific Journal Video about Chronic Constriction Injury of the Rat's Infraorbital Nerve IoN-CCI to Study Trigeminal Neuropathic Pain at JoVE.com

The authors acknowledge the work that was done by the first author of the IoN-CCI model, Bart Vos&#8224;.

伦理声明：动物治疗和关怀根据委员会的研究和IASP 11的伦理问题的指导方针。该协议被批准的机构伦理委员会。 1.主题<ol><li>使用雄性SD大鼠（250-300g的到港）。 </li><li>房子只在一个殖民地的房间有40-60％的湿度和21±1℃的室温固底笼。 </li><li>使水和食物提供不限量。 </li><li>保持大鼠下，反向12时12小时黑暗/光周期（开灯20小时）。 </li></ol> 2.手术...

<ol>
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离子的CCI模型中最令人感兴趣的方面是，它提供了自发的，非诱发（神经性）疼痛的唯一措施。神经性疼痛的大多数动物实验中，使用脊神经模型，专门解决nocifensive 行为 （例如，发声和戒断反应），反映过敏机械或热的刺激12。然而，患者的主要临床抱怨是持续性疼痛，痛觉过敏没有异常性疼痛或13。 本文章介绍了神经性疼痛以下离子CCI，孤立的脸?...

损坏的体感神经最常导致感觉丧失。它不很好地理解为什么和怎样，在一些情况下，外周神经损伤引起的慢性疼痛。其结果是，痛苦神经病仍然是具有挑战性的条件来治疗1和导致大幅度减少生活2-3的患者的生存质量。动物模型是研究的现有的和新开发的药物的疗效，并确定参与神经性疼痛的发展和维持的病理生理机制的一个重要工具。在对平移疼痛研究的主要挑战是确定和衡量自发性疼痛的动物（ 即 ，生理，行为或其他表现，表明疼痛的动物的存在是不被用探测被敏锐地叠加在对象的基础条件刺激的挑战）。此过程的总体目标是手术诱导眶下神经损伤的大鼠，导致两者自发的发展和诱发可用于研究参与三叉神经性疼痛和其治疗机制nocifensive行为。 导演，孤立的脸修饰是大鼠自发性神经病理性疼痛唯一的措施。以下眶下神经的慢性挤压伤（CCI）（离子），大鼠表现出变化自发行为和响应于触觉刺激的，指示的持续性疼痛和机械异常性疼痛4。以下离子的CCI，大鼠显示出增加的和非对称面的修饰活性， 即 ，与洗面笔划主要涉及神经损伤离子领土。与此相反的面的伤害性刺激（例如，福尔马林注射）5，非疼痛感觉障碍（即，单侧触须削波，麻药眶下神经阻滞，矿物油对VIBRI应用SSAE）没有或只有非常简要（即，发病后的第一分钟）诱导梳理行为6面对一个显著变化。得出的结论是离子CCI后，这种不正常的疏导是在受伤的神经境内自发的，强烈的厌恶感觉一种行为表现。在离子CCI大鼠这种行为带有很强的相似观察患者自发性疼痛。进一步的研究发现面部修饰行为之间的一个重要区别是一个更一般的身体修饰行为的一部分（ 即“身体美容在脸上疏导”），这在很大程度上仍然不受离子CCI和nocifensive面部美容既不之前也不随后体修饰（即，“分离的面部美容”），其中离子的CCI 7-9后显著增加。药理学上，在身体护理面部护理构成良好的控制测量非特异性药物作用（ <em&gt;例如，镇静）可能损害运动功能。在两种类型的脸的修饰的前肢运动是完全一样的。 以下离子的CCI，大鼠也响应于触觉刺激的，指示机械异常性疼痛4，10的呈现的改变。在第一周离子CCI后（多个），大鼠不再到轻度或中度触觉（即，的von Frey响应）的刺激。这种低反应，然后突然用一种极端的高反应性，从而即使是很微弱的刺激强度挑起nocifensive行为所取代。高反应性的可发性大鼠之间变化，但经过三至四周手术后最大鼠都成为高反应性。

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>Chromic catgut</td>
			<td>Dynek&#160;</td>
			<td>CG504</td>
			<td></td>
		</tr>
		<tr>
			<td>Nembutal&#160; (pentobarbital)</td>
			<td>Pharmacy</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Atropine</td>
			<td>Pharmacy</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Clippers for small animals - Favorita II</td>
			<td>Aesculap</td>
			<td>GT104</td>
			<td></td>
		</tr>
		<tr>
			<td>Stereotaxic instrument</td>
			<td>Stoelting</td>
			<td>51600</td>
			<td></td>
		</tr>
		<tr>
			<td>Ophthalmic ointment (Liposic)</td>
			<td>Pharmacy</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Homeothermic monitor system</td>
			<td>Harvard Apparatus</td>
			<td>PY8 50-7221-F</td>
			<td></td>
		</tr>
		<tr>
			<td>Cotton applicator</td>
			<td>Pharmacy</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Precision cotton swab</td>
			<td>Qosina</td>
			<td>10225</td>
			<td></td>
		</tr>
		<tr>
			<td>Operation microscope</td>
			<td>Kaps</td>
			<td>SOM 62</td>
			<td></td>
		</tr>
		<tr>
			<td>Dumont #5 forceps</td>
			<td>Fine Science Tools</td>
			<td>11251-10</td>
			<td></td>
		</tr>
		<tr>
			<td>Dumont forceps - Micro-blunted tips (#5/45)</td>
			<td>Fine Science Tools</td>
			<td>11253-25</td>
			<td></td>
		</tr>
		<tr>
			<td>Scissors - blunt tips</td>
			<td>Fine Science Tools</td>
			<td>14574-09</td>
			<td></td>
		</tr>
		<tr>
			<td>Polyester sutures - Mersilene (4-0)</td>
			<td>Ethicon</td>
			<td>R871H</td>
			<td></td>
		</tr>
		<tr>
			<td>Digital video camera</td>
			<td>Sony</td>
			<td>HDR-CX330E</td>
			<td></td>
		</tr>
		<tr>
			<td>Semmes-Weinstein Von Frey Aesthesiometer kit</td>
			<td>Stoelting</td>
			<td>58011</td>
			<td></td>
		</tr>
	</tbody>
</table>

chronic constriction injury of the rat's infraorbital nerve (ion-cci) to study trigeminal neuropathic pain

动物模型是研究的神经性疼痛的病理生理学和药理学的重要工具。这份手稿描述了外科手术和行为过程来研究三叉神经性疼痛的影响。满足三叉神经病疼痛综合症的特异性，眶下神经（离子）被松结扎神经经受慢性挤压伤（CCI）。一内部轨道的方式在这里呈现给揭露和结扎离子眶腔。离子结扎后，大鼠表现出变化自发行为和响应于的von Frey毛发刺激是表示持续性疼痛和机械异常性疼痛。两相可在的行为变化的发展来限定。在第一周离子CCI（阶段1），只显示出增加的和不对称的脸修饰活动， 即用洗面招主要针对神经损伤离子领土。它又分为脸部克之间进行ooming行为是更一般的身体梳理行为，这仍然基本不受离子的CCI的一部分，并且面对疏导既不是前面和后面由身体修饰，其中离子的CCI后显著增加。在此期间，响应于离子领土的机械刺激被降低。这种低反应突然由一个极端的高反应性，从而即使是很微弱的刺激强度惹nocifensive行为（第2阶段）所取代。这些行为改变和报道的面部疼痛征兆之间的相似性现象（即，响应于脸部的伤害性刺激）建议感觉迟钝/感觉异常和机械异常性疼痛在结扎离子领土的存在。

离子CCI大鼠表现出强烈的手术后增加的时间花费在分离面疏导（图1）的量。在第一手术后一周结束的增加的峰值，然后在接下来的数周内下降。最近的研究发现，面部修饰，以长达三个星期显著增加。在假手术组脸部美容的行为或多或少受到影响。 ION-CCI老鼠也表现出几乎完全没有反应的离子领土同侧机械刺激的第一个手术后一周（ 图2）中。在接?...

Watch this Scientific Journal Video about Chronic Constriction Injury of the Rat's Infraorbital Nerve IoN-CCI to Study Trigeminal Neuropathic Pain at JoVE.com

Chronic Constriction Injury of the Rat's Infraorbital Nerve IoN-CCI to Study Trigeminal Neuropathic Pain

This manuscript describes a rodent model to study trigeminal neuropathic pain. The methods described include surgical procedures to perform a chronic constriction injury of the rat&#8217;s infraorbital nerve and the post-surgical behavioral tests to measure the changes in spontaneous and evoked behavior that are indicative of persistent pain and allodynia.

老鼠的眶下神经（ION-CCI）研究三叉神经性疼痛性损伤

Animal models are important tools to study the pathophysiology and pharmacology of neuropathic pain. This manuscript describes the surgical and behavioral ...

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Medicine

Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain

Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats

慢性缩窄大鼠坐骨神经疼痛过敏测试

Chronic neuropathic pain, resulting from damage to the central or peripheral nervous system, is a prevalent and debilitating condition, affecting 7-18% of ...

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Breathing-controlled Electrical Stimulation (BreEStim) for Management of Neuropathic Pain and Spasticity

Breathing-controlled Electrical Stimulation BreEStim for Management of Neuropathic Pain and Spasticity

呼吸控制电气的刺激（BreEStim）的神经性疼痛和痉挛的管理

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Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice

膀胱胀诱发内脏运动反应为膀胱疼痛小鼠模型

Approximately 3-8 million people in the United States suffer from interstitial cystitis/bladder pain syndrome (IC/BPS), a debilitating condition ...

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Neuropathic pain arises as a consequence of a lesion or a disease affecting the somatosensory system. This syndrome results from maladaptive changes in ...

Facial Nerve Axotomy in Mice: A Model to Study Motoneuron Response to Injury

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Subcutaneous Trigeminal Nerve Field Stimulation for Refractory Facial Pain

皮下三叉神经场刺激难治性面部疼痛

Chronic or neuropathic trigeminal facial pain can be challenging to treat. Neurosurgical procedures should be applied when conservative treatment fails. ...

Development of Recombinant Proteins to Treat Chronic Pain

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Chronic pain is difficult to treat and new approaches to resolve persistent pain are urgently needed. Anti-inflammatory cytokines are promising candidates ...

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

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Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model

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傅氏皮下针刺治疗坐骨神经疼痛的疗效：慢性缩窄损伤大鼠模型中的行为和电生理变化

Fu&#39;s subcutaneous needling (FSN), an invented acupuncture technique from traditional Chinese medicine, is used worldwide for pain relief. However, the ...