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DOI :
10.3791/53179-v
December 1st, 2015
Chapters
0:05
Title
0:42
Mouse Mammary Gland Dissection
1:56
Mammary Tissue Fixation and Embedding
3:23
Frozen Tissue Sectioning
5:20
Indirect Immunofluorescence
8:47
Results: Mapping Proteins Involved with Lactation
9:53
Conclusion
在这篇文章中所描述的间接免疫荧光协议允许的检测和蛋白在小鼠乳腺定位。一个完整的方法是考虑到制备乳腺样品,利用荧光显微镜进行免疫组织化学,将图像的组织切片,并重建图像。
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