In Vivo SERCaMP Assay - AAV-GLuc-SERCaMP Injection
4:05
In Vivo SERCaMP Assay - Blood Sample Collection
5:45
Gaussia Luciferace Assay
6:39
Results: Endoplasm Calcium Homeostasis Assessment Using GLuc-SERCaMP
8:07
Conclusion
Endoplasmic reticulum calcium homeostasis is disrupted in diverse pathologies. A secreted ER calcium monitoring protein (SERCaMP) reporter can be used to detect disruptions in the ER calcium store. This protocol describes the use of a Gaussia luciferase SERCaMP to examine ER calcium homeostasis in vitro and in vivo.