October 8th, 2015
•This protocol compares the relative affinities of binding partners for Rho-family GTPases, including Rac1. In vivo, Rac1-binding proteins compete for a single binding interface, the conformation of which is dictated by a bound nucleotide. The nucleotide is both important and difficult to control experimentally, due to the high hydrolysis rate.
Related Videos
Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
Crystallization of Membrane Proteins in Lipidic Mesophases
High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
Identification of Protein Interacting Partners Using Tandem Affinity Purification
High-throughput Purification of Affinity-tagged Recombinant Proteins
GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved