Dissecting E7.5 or E8.5 Postimplantation Embryos for ex vivo Culture
2:03
Grafting Cultured Cells into E7.5 or E8.5 Mouse Embryos
3:42
Handmade Electroporation Materials and Apparatus Setup
5:19
Electroporation of E7.5 or E8.5 Embryos
6:44
Results: Localized Cell and DNA Transfer into Mouse Embryos
8:15
Conclusion
We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.