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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers

DOI :

10.3791/53369-v

September 29th, 2017

September 29th, 2017

9,879 Views

1University of Michigan Medical School, 2Department of Neurology, University of Michigan, 3Department of Internal Medicine, University of Michigan

This protocol uses three-dimensional (3D) imaging and analysis techniques to visualize and quantify nerve-specific mitochondria. The techniques are applicable to other situations where one fluorescent signal is used to isolate a subset of data from another fluorescent signal.

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Three dimensional Imaging

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