Crossing Transgenic Lines of Interest and Tamoxifen Induction
2:19
Combining Corneal Injury with Tamoxifen Induction
3:18
Preparing Tissue for Imaging
5:19
Confocal Microscopy and Image Analysis
6:04
Results: Corneal Lineage Tracing under Homeostasis and Injury
6:44
Conclusion
In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.