Mononuclear Cell (MNC) Isolation from Human Palatine Tonsils
2:45
Tonsillar T Lymphocyte Positive Selection
4:24
Isolated Tonsillar B and T Lymphocyte Flow Cytometric and PCR Analysis
5:39
Results: Representative Tonsillar B and T Lymphocyte Isolation
6:34
Conclusion
Palatine tonsils are a rich source of B and T lymphocytes. Here we provide an easy, efficient and rapid protocol to isolate B and T lymphocytes from human palatine tonsils. The method described has been specifically adapted for studies of the viral etiology of tonsil inflammation known as tonsillitis.