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8.9K Views
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06:31 min
December 12th, 2015
DOI :
10.3791/53437-v
Chapters
0:05
Title
0:35
Cotyledon specimen Sky Drop Mounting
2:35
Variable-angle Epifluorescence Microscopy (VAEM) Observation and Movie Acquisition
3:42
GFP-labeled Dot Residence Time Quantification by Kymograph Analysis
5:19
Results: Representative GFP-PATROL1 Dot Residence Time Analysis by Kymography
6:02
Conclusion
Summary
このプロトコルの目的は、気孔複合体の細胞皮質内に、GFPタグPATROL1、膜輸送タンパク質のドットを点滅示す可変角度落射蛍光顕微鏡で植物細胞表面上の蛍光標識タンパク質のダイナミクスを監視する方法を示すことですシロイヌナズナ 。
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