Name: long-term catheterization of the intestinal lymph trunk and collection of lymph in neonatal pigs
Uploaded: 2016-03-05T14:00:00
Description: Watch this Scientific Journal Video about Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs at JoVE.com

The work was supported in part by funding from Alberta Livestock and Meat Agency and Natural Science and Research Council Discovery grant to S. D. Proctor.

Все процедуры на экспериментальных животных, описанных в обоих видео и рукописи были утверждены по уходу и использованию комитета Institutional животных путем и следовал руководящим принципам, установленным Канадским советом по уходу за животными. 1. хирургической анестезии ?...

Сбор кишечника лимфы является отличным методом для изучения механизмов , участвующих в липида 8-12 и наркотиков 13-15 метаболизма, метастаз рака 16,17, незаконный оборот клеток и иммунной функции 18-26, в различных экспериментальных моделях на животных. Действительно, с?...

Лимфатическая система является изученной областью физиологии. Доклинические модели лимфатической катетеризацией встречаются у разных видов животных 1-8 и используются фармацевтической промышленности и научно - исследовательских институтов для изучения механизмов , участвующих в липида и 8-12 метаболизма лекарственных средств, 13-15 метастазирования рака 16 с экспериментального лечения 17 и иммунной функции 18 -26. Это исследование исследует использование кишечного лимфатического ствола катетеризацию в отечественной модели свиньи для измерения компонентов метаболизма липопротеинов. Метаболизм липопротеинов участвует в производстве и секреции хиломикронов, а также изменения в связанных липидов и общего белка. Это важные соображения, как существуют значительные различия в липидного обмена между наиболее часто используемых моделях грызунов и человека, и как таковые, с использованием модели свиньи для сбора кишечных лимфы может обеспечить более сопоставимую информацию для изучения липидного меняtabolism у людей 27-31. Несколько хирургических методов используются для сбора кишечника лимфу в крупных видов животных: черепную плечо подход (т.е. грудной проток катетеризацию) 5, боковая верхняя бочка подход 32-34 и вентральной средней линии или парамедианный подход 22,35. Это видео подробно описывает хирургическую процедуру в свиней с использованием вентральной средней линии хирургического подхода к катетеризацией кишечного лимфатического ствола. Осторожная хирургическая техника допускает этот метод лимфатической катетеризацией для сбора большого количества лимфы и его составные части в течение длительного периода времени. Этот метод открывает множество приложений для многих дисциплин, исследующих различные физиологические функции. Приложения могут включать в себя, но не ограничиваются ими, весь липопротеина тела и липидного обмена, иммунологического, генезис и метастазирования опухолей, функции кишечника, и Разработлор и прогрессирование воспалительного заболевания кишечника.

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long-term catheterization of the intestinal lymph trunk and collection of lymph in neonatal pigs

Catheterization of the intestinal lymph trunk in neonatal pigs is a technique allowing for the long-term collection of large quantities of intestinal (central) efferent lymph. Importantly, the collection of central lymph from the intestine enables researchers to study both the mechanisms and lipid constitutes associated with lipid metabolism, intestinal inflammation and cancer metastasis, as well as cells involved in immune function and immunosurveillance. A ventral mid-line surgical approach permits excellent surgical exposure to the cranial abdomen and relatively easy access to the intestinal lymph trunk vessel that lies near the pancreas and the right ventral segment of the portal vein underneath the visceral aspect of the right liver lobe. The vessel is meticulously dissected and released from the surrounding fascia and then dilated with sutures allowing for insertion and subsequent securing of the catheter into the vessel. The catheter is exteriorized and approximately 1 L/24 hr of lymph is collected over a 7 day period. While this technique enables the collection of large quantities of central lymph over an extended period of time, the success depends on careful surgical dissection, tissue handling and close attention to proper surgical technique. This is particularly important with surgeries in young animals as the lymph vessels can easily tear, potentially leading to surgical and experimental failure. The video demonstrates an excellent surgical technique for the collection of intestinal lymph.

<html> Лимфатической катетеризацию кишечника лимфатической ствола неонатальных свиней позволяет собирать около 1 л / 24 ч центральной лимфы в течение 7 дней. Лимфы, собранные в этом эксперименте содержали компоненты липидного обмена, а именно общего белка лимфы, ApoB48 липоп?...

Watch this Scientific Journal Video about Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs at JoVE.com

Long-Term Catheterization of the Intestinal Lymph Trunk and Collection of Lymph in Neonatal Pigs

We present a surgical procedure to catheterize the intestinal lymph trunk in neonatal pigs to collect large quantities of lipid metabolism components from efferent lymph.

Долгосрочное Катетеризация Кишечные лимфы Магистральные и коллекции лимфы в неонатальных Свиней

Catheterization of the intestinal lymph trunk in neonatal pigs is a technique allowing for the long-term collection of large quantities of intestinal ...

Lymphatic catheterization is an important technique used to investigate mechanisms involved in lipid and drug metabolism, cancer metastasis, and immune function and collecting large quantities of lymph from various animal models enables researchers to study these processes. The video demonstrates in detail the surgical procedure employed to catheterize the intestinal lymphatic trunk in neonatal pigs using a ventral mid-line approach. All procedures were approved by the University of Alberta Institutional Animal Care and Use Committee.To access the lymphatic vessel the abdominal cavity was opened. Electrocautery on the cut setting was used to open the muscle layer. A retractor was placed to hold the abdominal wall open for the duration of the procedure.Key abdominal organs are identified to aid in localizing the lymphatic vessel. For improved access to the lymphatic vessel segments of the small intestine and colon are exteriorized to the left side of the body. The lymphatic vessel is landmarked by identifying the liver and the right ventral segment of the portal vein.In neonatal pigs the lymphatic vessels are delicate and easily torn, so meticulous dissection with swabs and blunt instruments is required. Here is the intestinal lymphatic vessel. Now the suture is being passed underneath the lymphatic vessel with blunt forceps.Now there are three 2-0 silk sutures passed underneath the lymphatic vessel. The caudal most suture is used to dilate the vessel and secure the catheter to the vessel. The cranial sutures are used to secure the catheter within the vessel.A small incision in the lymphatic vessel allows blunt forceps to dilate the vessel. The catheter is inserted into the vessel. This must be done gently to ensure the lymphatic vessel does not tear.The catheter needs to be advanced beyond the cranial most suture to ensure it is well seated. The three 2-0 silk sutures secure the catheter into the lymphatic vessel. Importantly the two cranial sutures are first used to secure the catheter into the vessel and the caudal most suture is then used to tether the catheter, adding stability.It is important to ensure there is good lymph flow prior to closing the abdominal cavity. The catheter is exteriorized on the left mid-flank of the pig. Large forceps are used to pull the catheter from the abdominal cavity to the external surface of the pig's flank.The muscle layers are closed with 2-0 vicryl suture on a tapered needle in a simple interrupted suture pattern. The skin is closed with 2-0 vicryl suture on a cutting needle in a running subcuticular suture pattern. With the exit incision the catheter passes through the skin and is closed with 2-0 nylon suture on a cutting needle in a purse-string suture pattern.This closure helps prevent ascending infections tracking inwards along the catheter. The jacket holding the lymph collection bottles is placed on the pig while the pig is still under anesthesia. The catheter is inserted into the collection bottle and good lymph flow is assured prior to waking the pig.The pigs recover well from the procedure and excellent lymph flow is maintained throughout the experiment. The table displays the components of lymph collected from the lymphatic vessel. These components include Apolipoprotein B48, Triglycerides, and Cholesterol.The figure of the Western Blot shows the presence of both Apolipoprotein B48 and small amounts of plasma-derived Apolipoprotein B100 within the collected lymph sample. Although lymphatic catheterization in young pigs can be technically challenging, as the lymphatic vessels are both delicate and friable, this video demonstrates that using proper and careful surgical technique in young pigs can provide researchers with an excellent model for collecting intestinal lymph.

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Arrhythmias are common, affecting millions of patients worldwide. Current treatment strategies are associated with significant side effects and remain ...

Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer

Роботизированное тотальное мезоректальное иссечение и диссекция боковых тазовых лимфатических узлов при местно-распространенном раке прямой кишки средней и низкой

Since their approval for clinical use, da Vinci surgical robots have shown great advantages in gastrointestinal surgical operations, especially in complex ...