Yeast Cell Culture and Radiolabeling of Phosphoinositides
2:58
Deacylation and Extraction of the Radiolabeled Phosphoinositides
5:14
Separation of Tritium-labeled Glycero-inositides by High Pressure Liquid Chromatography (HPLC)
7:15
Data Analysis
8:55
Results: HPLC Analysis of Radiolabeled Phosphoinositides
9:55
Conclusion
Transcript
The overall goal of this procedure is to measure the amount of each phosphoinositide species in cells under various genetic and environmental conditions in order to understand phosphoinositide function and regulation. Phosphoinositide lipids contr
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Phosphoinositider signalerer lipider hvis relative overflod hurtigt skifter som reaktion på forskellige stimuli. Denne artikel beskriver en metode til at måle den overflod af phosphoinositider ved metabolisk mærkning celler med 3 H- myo-inositol, efterfulgt af ekstraktion og deacylering. Ekstraherede glycero-inositides adskilles derefter ved højtydende væskekromatografi og kvantificeres ved flow scintillation.