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Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

DOI :

10.3791/53552-v

April 2nd, 2016

April 2nd, 2016

13,764 Views

1Department of Cell and Systems Biology, University of Toronto

Experimental validation of enhancer activity is best approached by loss-of-function analysis. Presented here is an efficient protocol that uses CRISPR/Cas9 mediated deletion to study allele-specific regulation of gene transcription in F1 ES cells which contain a hybrid genome (Mus musculus129 x Mus castaneus).

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CRISPR Cas9

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