Immunostaining with Fluorescently Tagged Antibodies
7:30
Mounting Stained Cochlear Turns
8:59
Results: Examples of Optimal and Suboptimal Dissection and Immunostaining
10:54
Conclusion
Transcript
The overall goal of this whole mount dissection method is to isolate the sensory region of the calcified adult cochlea as three intact turns:the apex, middle, and base. This dissection method preserves the three dimensional structure of the organ
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Vi presenterer en metode for å isolere den voksne organ Corti som tre intakte cochlea svinger (apex, midtre og basen). Vi viser også en prosedyre for farging med fluorescently merkede antistoffer. Sammen disse teknikkene tillater studier av hårceller, støtte celler, og andre celletyper som finnes i sneglehuset.