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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

11.5K Views

09:16 min

October 11th, 2015

October 11th, 2015

11,541 Views

0:05

Title

0:54

Collecting Mouse Oocytes

2:08

Oocyte Microinjection

4:42

Culturing and Staining the Injected Oocytes

7:52

Results: Disruption of Pericentrin Expression in Oocytes during Meiotic Division

8:37

Conclusion

Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.

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