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DOI :
10.3791/53586-v
October 11th, 2015
Chapters
0:05
Title
0:54
Collecting Mouse Oocytes
2:08
Oocyte Microinjection
4:42
Culturing and Staining the Injected Oocytes
7:52
Results: Disruption of Pericentrin Expression in Oocytes during Meiotic Division
8:37
Conclusion
在这里,我们提出了一个协议,用于特定的siRNA介导的mRNA枯竭其次是免疫荧光分析来评估减数分裂纺锤体组装和组织在小鼠卵母细胞。这个协议是适合体外转录耗尽的和不同的主轴和/或微管组织中心相关因子中的卵母细胞的功能评价。
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