The overall goal of bioluminescent imaging is to image in vivo functional calcium transient, a proxy for activity, for longer periods, and in deep brain structures. This method can help answer key questions in the field of neurobiology, such as wh
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在这里,我们提出了一个新颖的钙利用生物发光记者-imaging方法。这种方法使用的稠构建GFP-水母发光蛋白,其结合的 Ca 2+并发光,省去了光激发。显著此方法允许长时间连续成像,获得脑深部的结构和高时间分辨率。