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DOI :
10.3791/53705-v
January 8th, 2016
Chapters
0:05
Title
0:57
Positioning and Gluing of Drosophila
2:23
Drosophila Dissection
3:58
Imaging Setup and Sample Preparation
5:47
Image Recording
7:39
Extracting Photon Values
9:51
Results: In Vivo Functional Brain Imaging
11:00
Conclusion
ここでは、生物発光レポーターを使用してアプローチを-imaging小説のCa 2+を提示します。このアプローチは、Ca 2+に結合し、励起光の必要性を排除し、光を発する融合構築物のGFPエクオリンを使用しています。重要なことは、この方法は、長い連続撮影、脳深部構造へのアクセス、高時間分解能を可能にします。
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