Radiation-attentuated Sporozoite Isolation from Mosquito Salivary Glands
2:07
Sporozoite Injection
3:13
Skin and Draining Lymphs Node Dissection
4:49
Cell Isolation
7:18
Results: Intradermal RAS Injection Increases Myeloid Cell Migration to Mouse Ear Skin and dLN
8:01
Conclusion
Transcript
The overall goal of this set of procedures is to immunize mice with radiation attenuated plasmodium parasites. To facilitate the analysis of myeloid cell recruitment in the mouse skin and draining lymph nodes. This method can help answer key quest
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We describe here a protocol for isolating myeloid cells from mouse skin and draining lymph node following intradermal injection of Plasmodium sporozoites. Flow cytometry of collected cells provides a reliable assay to characterize the skin and draining lymph node inflammatory response to the parasite.