Primary Leukemic Cell and Neutrophil Purity and Co-culture Phenotype Analysis by Flow Cytometry
7:58
3D Culture Setup and Phenotype Analysis
10:59
Results: Analysis of the Role of Neutrophils in Lymphoma Cell Sensitivity to Therapeutic Agents
13:09
Conclusion
Transcript
The overall goal of these procedures is to quickly isolate highly purified neutrophils from human blood samples by density gradient centrifugation to investigate their role in lymphoma cell sensitivity to cancer therapy using two D and three D cel
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Neutrophils are the most abundant type of white blood cells. The isolation of neutrophils from human blood by density gradient separation method and the differentiation of human promyelocytic (HL60) cells along the granulocytic pathway are described here; to test their role on sensitivity of lymphoma cells to anti-lymphoma agents.