Name: immunomagnetic separation of fat depot-specific sca1high adipose-derived stem cells (ascs)
Uploaded: 2016-08-11T14:00:00
Description: Watch this Scientific Journal Video about Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs at JoVE.com

Questo lavoro è sostenuto da NIH DK095137 (al THC). Ringraziamo i membri del laboratorio attuali ed ex che hanno contribuito allo sviluppo e alla sofisticazione dei metodi descritti.

Etica Dichiarazione: L&#39;Università del Michigan Comitato Uso e manutenzione degli animali (UCUCA) ha approvato tutti i metodi ei protocolli in conformità con la guida per la cura e l&#39;uso di animali da laboratorio (Institute for Research Laboratory Animal, Consiglio Nazionale delle Ricerche). I topi sono mantenuti in una Università del Michigan Vivarium e sono dati il ​​libero accesso a cibo e acqua e tenuti al / ciclo di luce 12 ore scuro. 1. preparati <ol><li> Preparare terr...

<ol>
	<li>Chun, T. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+pericellular+collagenase+directs+the+3-dimensional+development+of+white+adipose+tissue.">A pericellular collagenase directs the 3-dimensional development of white adipose tissue.</a> Cell. 125 (3), 577-591 (2006).</li><li>Chun, T. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+link+between+obesity+and+MMP14-dependent+adipogenic+collagen+turnover.">Genetic link between obesity and MMP14-dependent adipogenic collagen turnover.</a> Diabetes. 59 (10), 2484-2494 (2010).</li><li>Chun, T. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Peri-adipocyte+ECM+remodeling+in+obesity+and+adipose+tissue+fibrosis.">Peri-adipocyte ECM remodeling in obesity and adipose tissue fibrosis.</a> Adipocyte. 1 (2), 89-95 (2012).</li><li>Sun, K., Tordjman, J., Clement, K., Scherer, P. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fibrosis+and+adipose+tissue+dysfunction.">Fibrosis and adipose tissue dysfunction.</a> Cell Metab. 18 (4), 470-477 (2013).</li><li>Spangrude, G., Heimfeld, S., Weissman, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Purification+and+Characterization+of+Mouse+Hematopoietic+Stem+Cells.">Purification and Characterization of Mouse Hematopoietic Stem Cells.</a> Science. 241, 58-62 (1988).</li><li>Welm, B. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sca-1(pos)+cells+in+the+mouse+mammary+gland+represent+an+enriched+progenitor+cell+population.">Sca-1(pos) cells in the mouse mammary gland represent an enriched progenitor cell population.</a> Dev Biol. 245 (1), 42-56 (2002).</li><li>Gesta, S., Tseng, Y. H., Kahn, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Developmental+origin+of+fat:+tracking+obesity+to+its+source.">Developmental origin of fat: tracking obesity to its source.</a> Cell. 131 (2), 242-256 (2007).</li><li>Tang, W., Zeve, D., Suh, J. M., Bosnakovski, D., Kyba, M., Hammer, R. E., Tallquist, M. D., Graff, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=White+fat+progenitor+cells+reside+in+the+adipose+vasculature.">White fat progenitor cells reside in the adipose vasculature.</a> Science. 322, 583-586 (2008).</li><li>Rodeheffer, M. S., Birsoy, K., Friedman, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+white+adipocyte+progenitor+cells+in+vivo.">Identification of white adipocyte progenitor cells in vivo.</a> Cell. 135 (2), 240-249 (2008).</li><li>Tokunaga, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fat+depot-specific+gene+signature+and+ECM+remodeling+of+Sca1(high)+adipose-derived+stem+cells.">Fat depot-specific gene signature and ECM remodeling of Sca1(high) adipose-derived stem cells.</a> Matrix Biol. 36, 28-38 (2014).</li><li>Chun, T. H., Inoue, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3-D+adipocyte+differentiation+and+peri-adipocyte+collagen+turnover.">3-D adipocyte differentiation and peri-adipocyte collagen turnover.</a> Methods Enzymol. 538, 15-34 (2014).</li><li>Rajan, N., Habermehl, J., Cote, M. F., Doillon, C. J., Mantovani, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preparation+of+ready-to-use,+storable+and+reconstituted+type+I+collagen+from+rat+tail+tendon+for+tissue+engineering+applications.">Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications.</a> Nat Protoc. 1 (6), 2753-2758 (2006).</li><li>Ma, X., Robin, C., Ottersbach, K., Dzierzak, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Ly-6A+(Sca-1)+GFP+Transgene+is+Expressed+in+all+Adult+Mouse+Hematopoietic+Stem+Cells.">The Ly-6A (Sca-1) GFP Transgene is Expressed in all Adult Mouse Hematopoietic Stem Cells.</a> Stem Cells. 20 (6), 514-521 (2002).</li><li>Berry, R., Rodeheffer, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+the+adipocyte+cellular+lineage+in+vivo.">Characterization of the adipocyte cellular lineage in vivo.</a> Nat Cell Biol. 15 (3), 302-308 (2013).</li><li>Jeffery, E., Church, C. D., Holtrup, B., Colman, L., Rodeheffer, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+depot-specific+activation+of+adipocyte+precursor+cells+at+the+onset+of+obesity.">Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity.</a> Nat Cell Biol. 17 (4), 376-385 (2015).</li><li>Mori, S., Kiuchi, S., Ouchi, A., Hase, T., Murase, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characteristic+Expression+of+Extracellular+Matrix+in+Subcutaneous+Adipose+Tissue+Development+and+Adipogenesis;+Comparison+with+Visceral+Adipose+Tissue.">Characteristic Expression of Extracellular Matrix in Subcutaneous Adipose Tissue Development and Adipogenesis; Comparison with Visceral Adipose Tissue.</a> Int J Biol Sci. 10 (8), 825-833 (2014).</li><li>Ong, W. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+Specific+Cell-Surface+Markers+of+Adipose-Derived+Stem+Cells+from+Subcutaneous+and+Visceral+Fat+Depots.">Identification of Specific Cell-Surface Markers of Adipose-Derived Stem Cells from Subcutaneous and Visceral Fat Depots.</a> Stem Cell Reports. 2 (2), 171-179 (2014).</li></ol>

Qui dimostriamo l&#39;isolamento e la separazione immunomagnetica cellule di ASC murine da diversi cuscinetti di grasso e il loro utilizzo per esperimenti in vitro. Il metodo presentato è efficace per la rapida isolamento di un gran numero di ASC SCA1-positivo, che è vantaggiosa sulla tecnicamente complessi e costosi isolamento FACS-mediata di ASC 9,14. Diversamente FACS, separazione immunomagnetica non consente l&#39;uso di antigeni multipli per l&#39;identificazione di una popolazione di cellule ...

Staminali antigene cella 1 (Sca1, o Ly6A / E) per la prima volta identificata come un marcatore di superficie cellulare espressa da ematopoietiche e mesenchimali staminali cellule 5,6. La frazione vascolare stromale (SVF) di tessuto adiposo ottenuto da depositi di grasso del mouse è una popolazione eterogenea di cellule che compongono dei fibroblasti, macrofagi, cellule endoteliali vascolari, cellule neuronali e cellule progenitrici degli adipociti 7. Cellule progenitrici degli adipociti, o le cellule staminali derivate da tessuto adiposo (CSA) sono cellule non carichi di lipidi che si trovano nella matrice extracellulare perivascolare ricchi di collagene (ECM) 8. Circa il 50% della SVF costituiti da ASC, caratterizzati come lineage-negative (Lin -) e CD29 +: CD34 +: Sca1 + 9. La maggior parte di queste cellule sono Sca1 +: CD24 - progenitori adipociti, che sono capaci di adipociti differenziazione in vitro; Tuttavia, solo una frazione di cellule (0,08% del SVF) costituisce Sca1 <sup&gt; +: CD24 + cellule che sono pienamente in grado di proliferare e differenziarsi in adipociti nelle condizioni in vivo 9. Nonostante il potenziale avvertenza di usare Sca1 + SVF senza discriminare le cellule CD24 + CD24 da - cellule, isolando Sca1 + ASC da depositi di grasso che utilizzano la separazione immunomagnetica è un approccio efficiente e pratico per determinare il fenotipo delle cellule-autonoma di cellule progenitrici degli adipociti primaria. Nel campo di obesità e diabete, fibrosi dei tessuti e l&#39;infiammazione gioca un ruolo fondamentale nello sviluppo e nella manutenzione del diabete di tipo-2 3. Recentemente, Tokunaga et al. hanno dimostrato che le cellule di alta SCA1 isolate da inguinale (o sottocutanea, SQ) e perigonadal (o viscerale, VIS) depositi di grasso C57BL6 / J mostrano diverse firme genetiche e ECM rimodellamento in vitro 10. MMP14 (MT1-MMP), un membro prototipico della membrana-tmatrix ipo metalloproteinasi (MMP) famiglia media lo sviluppo di tessuto adiposo bianco (WAT), attraverso la sua attività collagenolitica 1. Esempi di esperimenti che possono essere eseguite con le cellule isolate e arricchite attraverso il seguente protocollo includono cultura tridimensionale, studi di differenziazione, saggi di degradazione del collagene, e RNA sequenziamento 10,11. Test di degradazione devono essere condotte con il collagene acido estratto per garantire la conservazione di telopeptide 11,12. Il seguente protocollo dimostrerà i metodi per isolare le cellule stromali vascolari primarie da diversi depositi di grasso e di arricchire le cellule progenitrici degli adipociti utilizzando separazione immunomagnetica. La validità della separazione delle cellule sarà valutata con la citometria a flusso e attraverso l&#39;utilizzo di topi Sca1-GFP che esprimono GFP in Sca1 + cellule, guidata da un promotore Sca1 13.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Type 3 Collagenase</td>
			<td>Worthington Biochemical</td>
			<td>LS004182</td>
			<td>Tissue digestion</td>
		</tr>
		<tr>
			<td>DMEM</td>
			<td>Gibco</td>
			<td>11965-092</td>
			<td>High-glucose culture medium</td>
		</tr>
		<tr>
			<td>Pen/Strep/Glutamine (100x)</td>
			<td>Gibco</td>
			<td>10378-016</td>
			<td>Media antibiotic</td>
		</tr>
		<tr>
			<td>Anti-anti (100x)</td>
			<td>Gibco</td>
			<td>15240-062</td>
			<td>Media antifungal</td>
		</tr>
		<tr>
			<td>FBS</td>
			<td>Gibco</td>
			<td>16000-044</td>
			<td></td>
		</tr>
		<tr>
			<td>PBS (1x, pH 7.4)</td>
			<td>Gibco</td>
			<td>10010-023</td>
			<td></td>
		</tr>
		<tr>
			<td>Trypsin (0.05%)</td>
			<td>Gibco</td>
			<td>25300-054</td>
			<td></td>
		</tr>
		<tr>
			<td>Cell strainer</td>
			<td>BD Bioscience</td>
			<td>352360</td>
			<td>100-&#956;m cell strainer</td>
		</tr>
		<tr>
			<td>60mm plates</td>
			<td>BD Falcon</td>
			<td>353004</td>
			<td></td>
		</tr>
		<tr>
			<td>Scissors</td>
			<td>FST</td>
			<td>14001-12</td>
			<td>Large</td>
		</tr>
		<tr>
			<td>Scissors</td>
			<td>FST</td>
			<td>14091-11</td>
			<td>Fine, curved tip</td>
		</tr>
		<tr>
			<td>Large Forceps</td>
			<td>FST</td>
			<td>11000-12</td>
			<td></td>
		</tr>
		<tr>
			<td>Fine Forceps</td>
			<td>Any vendor</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>25G 5/8&#8221; needles</td>
			<td>BD</td>
			<td>305122</td>
			<td></td>
		</tr>
		<tr>
			<td>22G 1.5&#8221; needles</td>
			<td>BD</td>
			<td>305159</td>
			<td></td>
		</tr>
		<tr>
			<td>15 ml conical tubes</td>
			<td>BD Falcon</td>
			<td>352097</td>
			<td></td>
		</tr>
		<tr>
			<td>50 ml conical tubes</td>
			<td>BD Falcon</td>
			<td>352098</td>
			<td></td>
		</tr>
		<tr>
			<td>MACS separation columns</td>
			<td>Miltenyi Biotec</td>
			<td>130-042-201</td>
			<td></td>
		</tr>
		<tr>
			<td rowspan="3">Anti-Sca1 microbead kit (FITC)</td>
			<td rowspan="3">Miltenyi Biotec</td>
			<td rowspan="3">130-092-529</td>
			<td rowspan="3">FITC-anti-Sca1 1&#186;Ab and anti-FITC microbeads 2&#186;Ab</td>
		</tr>
		<tr>
		</tr>
		<tr>
		</tr>
		<tr>
			<td>AutoMACS running buffer</td>
			<td>Miltenyi Biotec</td>
			<td>130-091-221</td>
			<td></td>
		</tr>
		<tr>
			<td>MiniMACS separator</td>
			<td>Miltenyi Biotec</td>
			<td>130-042-102</td>
			<td></td>
		</tr>
		<tr>
			<td>MACS MultiStand</td>
			<td>Miltenyi Biotec</td>
			<td>130-042-303</td>
			<td></td>
		</tr>
		<tr>
			<td>Blue chux pads</td>
			<td>Fisher</td>
			<td>276-12424</td>
			<td></td>
		</tr>
		<tr>
			<td>Absorbent pads</td>
			<td>Fisher</td>
			<td>19-165-621</td>
			<td></td>
		</tr>
		<tr>
			<td>Styrofoam board</td>
			<td></td>
			<td></td>
			<td>Use from 50ml tubes</td>
		</tr>
		<tr>
			<td>70% ethanol</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane</td>
			<td>Any vendor</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Rat IgG2a Alexa Fluor 647</td>
			<td>Invitrogen</td>
			<td>R2a21</td>
			<td></td>
		</tr>
		<tr>
			<td>Rat IgG2a anti-mouse Sca1 Alexa Fluor 647</td>
			<td>Invitrogen</td>
			<td>MSCA21</td>
			<td></td>
		</tr>
		<tr>
			<td>Rat IgG2a R-PE</td>
			<td>Invitrogen</td>
			<td>R2a04</td>
			<td></td>
		</tr>
		<tr>
			<td>Rat IgG2a anti-mouse F4/80 R-PE</td>
			<td>Invitrogen</td>
			<td>MF48004</td>
			<td></td>
		</tr>
		<tr>
			<td>Round-bottom tube</td>
			<td>BD Falcon</td>
			<td>352058</td>
			<td></td>
		</tr>
		<tr>
			<td>HBSS (&#8211;Ca, &#8211;Mg)</td>
			<td>Gibco</td>
			<td>14175-095</td>
			<td></td>
		</tr>
		<tr>
			<td>HBSS (+Ca, +Mg)</td>
			<td>Gibco</td>
			<td>14025-092</td>
			<td>For collagenase solution</td>
		</tr>
		<tr>
			<td>Type I collagen (2.7 mg/ml in 37mm acetic acid</td>
			<td>Prepare in house12</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>10x MEM</td>
			<td>Gibco</td>
			<td>11430-030</td>
			<td></td>
		</tr>
		<tr>
			<td>1M HEPES</td>
			<td>Gibco</td>
			<td>15630-080</td>
			<td></td>
		</tr>
		<tr>
			<td>0.34N NaOH</td>
			<td>Prepare in house</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Cover slips</td>
			<td>Corning</td>
			<td>2870-22</td>
			<td></td>
		</tr>
		<tr>
			<td>Alexa Fluor 594 carboxylic acid, succinimidyl ester, mixed isomers</td>
			<td>Invitrogen</td>
			<td>A-20004</td>
			<td></td>
		</tr>
		<tr>
			<td>0.89M NaHCO3&#160;</td>
			<td>Gibco</td>
			<td>25080-094</td>
			<td></td>
		</tr>
	</tbody>
</table>

immunomagnetic separation of fat depot-specific sca1high adipose-derived stem cells (ascs)

The isolation of adipose-derived stem cells (ASCs) is an important method in the field of adipose tissue biology, adipogenesis, and extracellular matrix (ECM) remodeling. In vivo, ECM-rich environment consisting of fibrillar collagens provides a structural support to adipose tissues during the progression and regression of obesity. Physiological ECM remodeling mediated by matrix metalloproteinases (MMPs) plays a major role in regulating adipose tissue size and function1,2. The loss of physiological collagenolytic ECM remodeling may lead to excessive collagen accumulation (tissue fibrosis), macrophage infiltration, and ultimately, a loss of metabolic homeostasis including insulin resistance3,4. When a phenotypic change of the adipose tissue is observed in gene-targeted mouse models, isolating primary ASCs from fat depots for in vitro studies is an effective approach to define the role of the specific gene in regulating the function of ASCs. In the following, we define an immunomagnetic separation of Sca1high ASCs.

Arricchimento di alte ASC SCA1 provenienti da diversi cuscinetti di grasso. Le cellule stromali vascolari isolati dalla visualizzazione grasso SQ fibroblasti-like, allungato la forma delle cellule indipendentemente dal livello di espressione Sca1 (Figura 1A). D&#39;altra parte, VIS (EWAT-derivati) SCA1 alta e bassa Sca1 cellule mostrano netta differenza nella loro forma cellula...

Watch this Scientific Journal Video about Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs at JoVE.com

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs

We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.

Separazione immunomagnetica di Sca1 Fat Depot-specifica<sup&gt; alta</sup&gt; derivate da tessuto adiposo cellule staminali (ASC)

The isolation of adipose-derived stem cells (ASCs) is an important method in the field of adipose tissue biology, adipogenesis, and extracellular matrix ...

Research

JoVE Journal

Developmental Biology

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Isolamento e arricchimento di Human derivate da tessuto adiposo cellule stromali di Osteogenesi avanzata

Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are considered the gold standard for stem cell-based tissue engineering applications. However, the ...

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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Isolamento e differenziazione delle cellule staminali derivate da tessuto adiposo sottocutaneo da Suini tessuti adiposi

Obesity is an unconstrained worldwide epidemic. Unraveling molecular controls in adipose tissue development holds promise to treat obesity or diabetes. ...

An Efficient Method to Obtain Dedifferentiated Fat Cells

Un metodo efficiente per ottenere cellule di grasso dedifferenziato

Tissue engineering and cell therapy hold great promise clinically. In this regard, multipotent cells, such as mesenchymal stem cells (MSCs), may be used ...

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Condrogenico Differenziazione induzione di cellule staminali derivate da tessuto adiposo per gravità centrifuga

Impaired cartilage cannot heal naturally. Currently, the most advanced therapy for defects in cartilage is the transplantation of chondrocytes ...

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

Isolamento rapido di BMPR-IB + derivate da tessuto adiposo cellule stromali per l&#39;uso in un cranica difetto di guarigione Modello

Invasive cancers, major injuries, and infection can cause bone defects that are too large to be reconstructed with preexisting bone from the patient's own ...

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

Una tecnica novel di trapianto di grassi del grasso mammario per visualizzare la generazione di vascelli delle cellule staminali endoteliali vascolari

Endothelial cells (ECs) are the fundamental building blocks of the vascular architecture and mediate vascular growth and remodeling to ensure proper ...

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Supporto orizzontale intero: un protocollo di elaborazione e imaging novello per le sezioni trasversali tessute spesse e tridimensionali della pelle

Processing a tissue of interest to generate a microscopic image that supports a scientific argument can be challenging. The acquisition of high-quality ...

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

In Vivo Rilevamento di umani cellule staminali mesenchimali adiposo-derivate in un modello di osteoartrite del ginocchio di ratto con colorante fluorescente lipofilico membrana

In order to support the clinical application of human adipose-derived mesenchymal stem cell (haMSC) therapy for knee osteoarthritis (KOA), we examined the ...

Single-cell Photoconversion in Living Intact Zebrafish

Cella singola fotoconversione in Zebrafish intatto vivente

Animal and plant tissue is composed of distinct populations of cells. These cells interact over time to build and maintain the tissue and can cause ...

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Analisi computazionale della linea germinale Caenorhabditis elegans di studiare la distribuzione dei Nuclei, le proteine ed il citoscheletro

The Caenorhabditis elegans (C. elegans) germline is used to study several biologically important processes including stem cell development, apoptosis, and ...