Name: simultaneous detection of c-fos activation from mesolimbic and mesocortical dopamine reward sites following naive sugar and fat ingestion in rats
Uploaded: 2016-08-24T13:00:00
Description: Watch this Scientific Journal Video about Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats at JoVE.com

由于戴安娜卡萨-Culaki，克里斯塔尔桑普森和神学Karagiorgis为他们在这个项目上努力工作。

这些实验方案已获得批准的机构动物护理和使用委员会证明所有的主体和程序均符合健康指南护理全国学院和实验动物使用。 1.主题<ol><li>购买和/或品种雄性SD大鼠（260 - 300克）。 </li><li>房子只单独在铁丝网笼子里。保持他们与大鼠饲料和水可随意 12:12小时的光/暗周期。 </li><li>分配适当的样本大小（例如 ，N≈6 - 8）随机分组。 </li></ol> 2.测试仪器和收容程?...

这项研究的目的是利用细胞的c-fos的技术来确定源（VTA）和前脑投射目标DA奖励相关的神经元（NAC，AMY，内侧前额叶皮质）的脂肪和糖的新型摄入后同时激活大鼠。本研究是此前69发表的一项研究协议的详细描述。据推测，在VTA，其主要投影区域的前度和infralimbic mPFC的，在NAC的核和壳和基底外侧和中央皮质内侧AMY，以及背侧纹状体将充当分布式脑网络24 -27，并显示协调下同时FLI小?...

脑内多巴胺（DA）已通过牵连到可口的糖的摄入量响应中央提出的享乐1,2，努力有关的3和习惯为基础的行动4,5机制。在这些效应牵连主DA通路起源于腹侧被盖区（VTA），和项目到伏隔核（NAC）芯和壳，基底外侧和中央皮质内侧杏仁核（AMY），以及前度和infralimbic内侧前额叶皮层（内侧前额叶皮质）（查看评论6,7）。该VTA有牵连的蔗糖摄入量8,9，和DA释放后观察糖的摄入量在10-15 NAC，AMY 16,17和内侧前额叶皮质18-20。脂肪的摄入量也刺激多巴胺释放的NAC 21，另有丰富DA投影带的背侧纹状体（尾状核）已与DA介导的喂养22,23也被相关。凯利24-27提出，这些多项目该DA介导系统的离子区形成通过广泛而亲密互连28-34集成和交互的分布式网络的大脑。 除了​​DA D1和D2受体拮抗剂来减少糖35-37和脂肪38-40的摄取的能力，DA信令也已牵涉于介导糖和脂肪以产生调节味道偏好（CFP）的能力41- 46。一个DA D1受体拮抗剂进入NAC，AMY或内侧前额叶皮质47-49显微注射消除CFP的收购胃内引起的葡萄糖。而无论是DA D1或D2受体拮抗剂显微注射到内侧前额叶皮质消除收购果糖CFP 50，收购和果糖CFP表达的差异由NAC和AMY 51,52 DA受体拮抗剂阻断。 在c-fos技术53,54已被用来研究神经活化法制由可口的摄入和神经激活ñ诱导。术语“C-fos的激活”，将整个手稿中使用，并且通过增加的c-Fos蛋白的转录神经元去极化期间可操作限定。蔗糖摄取在中央AMY核增加fos的样免疫反应（FLI），所述VTA以及外壳，但不是核心，在NAC 55-57的。而糖水摄取量假喂养大鼠的AMY和NAC显著上升FLI，但不是VTA 58，胃内蔗糖或葡萄糖输注显著的NAC和AMY 59,60中央和基底外侧核增加FLI。重复加入蔗糖至预定食物访问在mPFC的增加FLI以及在NAC壳和芯61。蔗糖浓度降档模式显示，最大的FLI增加发生在基底AMY和NAC，但不是VTA 62。继空调，糖有关的自然雷瓦灭绝RD行为的基底AMY和NAC 63增加FLI。此外，配对糖可用性达到了口气导致口气随后在基底64 AMY增加FLI水平。高脂肪的摄入量也NAC和内侧前额叶皮质部位增加65-67 FLI。 大多数先前引用的研究中单个位点，不提供关于奖赏相关分布式大脑网络24-27的识别信息检测上的c-fos的激活糖和脂肪的效果。此外，许多研究也没有描绘在NAC（核和壳），AMY（基底和中央皮质内侧）和内侧前额叶皮质的子区域的相对贡献（前度和infralimbic）可能潜在的被检查优势出色的空间，在C-FOS映射68个单细胞的决议。我们的实验室69最近使用的c-fos的激活和同时测量改建的VTA DA通路及其亲jection区（NAC，AMY和内侧前额叶皮质）大鼠脂肪和糖的摄入小说之后。本研究描述的程序和方法步骤，同时分析是否急性暴露六个不同的解决方案（玉米油，葡萄糖，果糖，糖精，水和脂肪乳剂控制）在NAC，AMY的子区域将差异激活FLI，内侧前额叶皮质以及背侧纹状体。这种差异同时检测系统允许的每个站点和决心FLI显著效果的确认，是否与相关网站的变化密切相关，从而为分布式网络的脑支持24-27日在一个特定的站点变化。这些经过测试的程序是否VTA中，前度和infralimbic内侧前额叶皮质的NAC的核心和壳，以及基底和中央皮质，内侧AMY）以及背侧纹状体会显示协调，口服后，无条件的摄入同时FLI激活葡萄糖（8％），果糖（8％），玉米油（3.5％）和糖精（0.2％）的解决方案。

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simultaneous detection of c-fos activation from mesolimbic and mesocortical dopamine reward sites following naive sugar and fat ingestion in rats

本研究采用细胞的c-fos的激活来评估脂肪和糖对大鼠脑内多巴胺（DA）的新途径摄入的影响。糖和脂肪摄入量是由他们天生的景点，以及了解到的喜好调节。脑多巴胺，特别是内消旋 - 边缘和从腹侧被盖区（VTA）内消旋 - 皮质突起，已经牵涉在这两个未学习并了解到响应。分布式脑网络，其中的几个位点和发射机/肽系统交互的概念，已提出​​调解适口食物摄入量，但有证据有限经验证明这种行动。因此，糖的摄入引起从个体VTA多巴胺投影区，包括伏隔核（NAC），杏仁核（AMY）DA释放和增加的c-fos的样免疫反应（FLI）和内侧前额叶皮质（mPFC的）以及背侧纹状体。此外，选择性多巴胺受体拮抗剂的集中管理到这些网站Ş差异减少糖类和脂肪引起的空调味的喜好采集和表达。一种方法，借以确定这些网站是否响应糖或脂肪摄入互动作为分布式脑网络将同时评估是否对VTA及其主要mesotelencephalic DA投影区域（前度和伏隔核的infralimbic内侧前额叶皮质，核和壳，基底外侧和中央皮质，内侧AMY）以及背侧纹状体会显示协调，口服后，无条件的摄入玉米油（3.5％），葡萄糖（8％），果糖（8％）和糖精的同时FLI激活（0.2 ％）的解决方案。这种方法在鉴别同时使用到相关的大脑部位细胞的c-fos的激活，研究中啮齿类动物的可口食物摄取奖励有关学习的可行性了成功的第一步。

下面描述的所有代表性结果先前已发表​​69，并在此处重新提出支持“概念验证”中指示的技术的有效性。 解决方案摄入量 在基线糖精摄入显著差异观察到在第一四天所有动物（F（3108）= 57.27，P &lt;0.001）与摄入量（第1天：1.3（±0.2）毫升;第2天：3.9（±0.4）毫升;第3天：5.9（±0.6）毫升;第4天：7.1（...

Watch this Scientific Journal Video about Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats at JoVE.com

Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats

这项研究的目的是通过使用蜂窝的c-fos的激活来测量大鼠的脂肪和糖新颖摄入后多巴胺途径和终端站点同步变化划定一个可靠免疫组织学技术，以确定奖励相关的分布式脑网络。

从脑边缘和Mesocortical多巴胺奖赏网站继朴素的糖和脂肪摄入大鼠c-fos激活的同时检测

This study uses cellular c-fos activation to assess effects of novel ingestion of fat and sugar on brain dopamine (DA) pathways in rats. Intakes of sugars ...

Research

JoVE Journal

Neuroscience

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科研

神经科学

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