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Neuroscience

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Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats

8.9K Views

08:07 min

August 24th, 2016

August 24th, 2016

8,918 Views

0:05

Title

0:54

Testing Apparatus and Intake Procedures

2:06

Tissue Preparation

3:22

c-fos Staining Procedure

5:29

Determination of c-fos Immunoreactive Counts

6:45

Results: Actual Amygdala and Ventral Tegmental Area c-fox Activation Following Fats and Sugars

7:35

Conclusion

Transcript

The overall goal of this experimental protocol is to determine whether the VTA, and it's major mesotelencephatic DA projection zones would display coordinate and simultaneous c-Fos activation after novel intake of corn oil, glucose, fructose, or s

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The goal of this study is to identify reward-related distributed brain networks by delineating a reliable immunohistological technique using cellular c-fos activation to measure simultaneous changes in dopamine pathways and terminal sites after novel ingestion of fat and sugar in rats.

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