La inducción de la encefalomielitis autoinmune experimental en ratones y evaluación de la distribución de la enfermedad dependiente de las células inmunes en diversos tejidos

This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.