Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

16.9K Views

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09:27 min

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April 22nd, 2016

DOI :

10.3791/53944-v

April 22nd, 2016

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16,940 Views

Hui Gao¹, Eshita Khera², Jung-Kul Lee¹, Fei Wen²

¹Department of Chemical Engineering, Konkuk University, ²Department of Chemical Engineering, University of Michigan

Chapters

0:05

Title

0:45

Whole-cell Biocatalysts Preparation

2:05

Biosynthesis of L-xylulose by Coupling E. coli_HjLAD and E. coli_SpNOx for Cofactor Regeneration

3:51

Immobilization of Recombinant Whole-cell Catalysts in Calcium Alginate Beads

6:25

Stability Assay of Immobilized Biocatalysts for L-xylulose Production

7:15

Results: Co-immobilization of Whole-cell Biocatalysts Improves Reusability

8:55

Conclusion

Transcript

The overall goal of this experimental procedure is to demonstrate the use of a coupled whole cell biocatalytic system immobilized in alginate beads for improved production yield and reusability. This is my subject, I have answered key questions in

Summary

Presented is the protocol for co-immobilizing whole-cell biocatalysts for cofactor regeneration and improved reusability, using the production of L-xylulose as an example. The cofactor regeneration is achieved by coupling two Escherichia coli strains expressing functionally complementary enzymes; the whole-cell biocatalyst immobilization is achieved by cell encapsulation in calcium alginate beads.

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Keywords Immobilization

Multi biocatalysts

Alginate Beads

Cofactor Regeneration

Reusability

Whole Cell Biocatalytic System

Biocatalysts

Stability

Cofactor Regenerating

E Coli

SpNOx