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DOI :
10.3791/53944-v
April 22nd, 2016
Chapters
0:05
Title
0:45
Whole-cell Biocatalysts Preparation
2:05
Biosynthesis of L-xylulose by Coupling E. coliHjLAD and E. coliSpNOx for Cofactor Regeneration
3:51
Immobilization of Recombinant Whole-cell Catalysts in Calcium Alginate Beads
6:25
Stability Assay of Immobilized Biocatalysts for L-xylulose Production
7:15
Results: Co-immobilization of Whole-cell Biocatalysts Improves Reusability
8:55
Conclusion
提示例としてL-キシルロースの生産を使用して、補因子再生と改善された再利用のための共固定化全細胞生体触媒のためのプロトコルです。補因子再生の機能的相補酵素を表す2 大腸菌株を結合することによって達成されます。全細胞生体触媒の固定化は、カルシウムアルギネートビーズ中の細胞のカプセル化により達成されます。
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