Evaluation of the Purity of Isolated Primary Villous Cytotrophoblasts
3:13
siRNA Transfection of Human Primary Villous Cytotrophoblasts
4:55
Preparation of Nuclear Extracts from Cultured Cells
5:52
Radiolabeling of Single Stranded Oligonucleotide
6:50
DNA Binding Reaction
8:07
Results: Syncytin-2 Knowckdown and Visualization of Syncytin-2 DNA-protein Complexes
9:14
Conclusion
Transcript
The overall goal of this protocol is to demonstrate optimal conditions for the transfection of freshly isolated primary villous cytotrophoblasts and to present a method for the identification of DNA protein interaction at promoter sites. This meth
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Denne protokol beskriver en fremgangsmåde til effektiv transfektion af siRNA i frisk isolerede humane villøse cytotrophoblaster hjælp mikroperforering og identificere DNA-protein-komplekser i disse celler. Transficerede celler kan monitoreres ved Western blot og EMSA-analyser under 4-dages dyrkningstid.