Evaluation of the Purity of Isolated Primary Villous Cytotrophoblasts
3:13
siRNA Transfection of Human Primary Villous Cytotrophoblasts
4:55
Preparation of Nuclear Extracts from Cultured Cells
5:52
Radiolabeling of Single Stranded Oligonucleotide
6:50
DNA Binding Reaction
8:07
Results: Syncytin-2 Knowckdown and Visualization of Syncytin-2 DNA-protein Complexes
9:14
Conclusion
Transcript
The overall goal of this protocol is to demonstrate optimal conditions for the transfection of freshly isolated primary villous cytotrophoblasts and to present a method for the identification of DNA protein interaction at promoter sites. This meth
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Ce protocole décrit un procédé pour transfecter efficacement ARNsi dans les cytotrophoblastes villeux humaines fraîchement isolées en utilisant la microporation et l'identification des complexes ADN-protéine dans ces cellules. Les cellules transfectées peuvent être contrôlées par Western Blot et des analyses EMSA pendant la période de culture de 4 jours.