Evaluation of the Purity of Isolated Primary Villous Cytotrophoblasts
3:13
siRNA Transfection of Human Primary Villous Cytotrophoblasts
4:55
Preparation of Nuclear Extracts from Cultured Cells
5:52
Radiolabeling of Single Stranded Oligonucleotide
6:50
DNA Binding Reaction
8:07
Results: Syncytin-2 Knowckdown and Visualization of Syncytin-2 DNA-protein Complexes
9:14
Conclusion
Transcript
The overall goal of this protocol is to demonstrate optimal conditions for the transfection of freshly isolated primary villous cytotrophoblasts and to present a method for the identification of DNA protein interaction at promoter sites. This meth
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이 프로토콜은 효과적으로 격리 갓 인간 융모 cytotrophoblasts에 siRNA를 형질 감염 microporation을 사용하여 이들 세포의 DNA 단백질 복합체를 식별하는 방법을 설명한다. 형질 감염된 세포는 웨스턴 블롯에 의해 모니터링 될 수 있고, EMSA는 4 일간의 배양 기간 동안 분석한다.