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DOI :
10.3791/54041-v
June 2nd, 2016
Chapters
0:05
Title
0:58
Influenza H3N2 Virus-like Particle (VLP) Generation
2:10
CHIK VLP Expression Using Baculovirus/Insect Cell System
3:18
Sedimentation and Purification of VLPs and SVPs
4:42
Results: Successful Expression and Purification of VLPs
5:23
Conclusion
ここでは、バキュロウイルスまたは哺乳動物発現系、および超遠心分離精製のいずれかを使用してウイルス様粒子を合成するためのプロトコルを提示します。この高度にカスタマイズ可能なアプローチは、安全かつ柔軟な方法で、ワクチン標的としてウイルス抗原を同定するために使用されます。
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